RNA and DNA Editing

Methods and Protocols

  • Ruslan Aphasizhev

Part of the Methods in Molecular Biology book series (MIMB, volume 718)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Uracil Insertion/Deletion RNA Editing in Mitochondrion of Trypanosoma brucei

    1. Front Matter
      Pages 1-1
    2. H. Ulrich Göringer, Holger Stark, Cordula Böhm, Bjoern Sander, Monika M. Golas
      Pages 3-22
    3. Gene-Errol Ringpis, Richard H. Lathrop, Ruslan Aphasizhev
      Pages 23-37
  3. Adenosine to Inosine RNA Editing

    1. Front Matter
      Pages 39-39
    2. Cynthia J. Staber, Selena Gell, James E. C. Jepson, Robert A. Reenan
      Pages 41-73
    3. Hui Sun, Aruna Raja, Mary A. O’Connell, Valerie Mann, Brendon Noble, Liam P. Keegan
      Pages 75-87
  4. Cytidine to Uracil RNA and DNA Editing

    1. Front Matter
      Pages 101-101
    2. Brad R. Rosenberg, Scott Dewell, F. Nina Papavasiliou
      Pages 103-119
    3. Valerie Blanc, Nicholas O. Davidson
      Pages 121-135
    4. Stephanie Ruf, Ralph Bock
      Pages 137-150
    5. Meng Wang, Cristina Rada, Michael S. Neuberger
      Pages 171-184
  5. tRNA Editing and RNA Modifications

  6. Back Matter
    Pages 271-275

About this book


The recent expansion in diversity of RNA and DNA editing types has stimulated the development of many unique genetic, molecular, biochemical, and computational approaches to biological issues.  In RNA and DNA Editing: Methods and Protocols, leading experts in the field introduce methods developed over the last few years to study editing substrates, mechanisms of specificity, and functions of RNA and DNA editing enzymes and complexes. Sections of the book are dedicated to state-of-the art techniques which enable investigation of uracil insertion/deletion RNA editing in mitochondrion of Trypanosoma brucei, adenosine to inosine RNA editing, cytidine to uracil RNA and DNA editing, as well as tRNA editing and RNA modifications.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.


Authoritative and cutting-edge, RNA and DNA Editing: Methods and Protocols seeks to inspire the further development of these vital and powerful techniques.


Adenosine to inosine editing Anti-viral defense Antibody gene diversification Cytidine RNA nucleotide sequence RNA polymerase stuttering RNA-directed cascades Uracil insertion and deletion tRNA editing

Editors and affiliations

  • Ruslan Aphasizhev
    • 1
  1. 1.School of Medicine, Dept. Microbiology & Molecular GeneticsUniversity of California, IrvineIrvineUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-017-1
  • Online ISBN 978-1-61779-018-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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