Plant Embryo Culture

Methods and Protocols

  • Trevor A. Thorpe
  • Edward C. Yeung

Part of the Methods in Molecular Biology book series (MIMB, volume 710)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Culture of Zygotic Embryos: Fundamental Studies

    1. Front Matter
      Pages 1-1
    2. Tegan M. Haslam, Edward C. Yeung
      Pages 3-15
    3. Nicole S. Ramesar-Fortner, Edward C. Yeung
      Pages 29-37
    4. Florent Engelmann, Bernard Malaurie, Oulo N’Nan
      Pages 63-72
  3. Application of Embryo Culture Techniques: Embryo Rescue

    1. Front Matter
      Pages 73-73
    2. Xiuli Shen, Fred G. Gmitter Jr., Jude W. Grosser
      Pages 75-92
    3. Nalini Mallikarjuna, Fred J. Muehlbauer
      Pages 93-105
    4. Hector Gordon Nuñez-Palenius, Rafael Ramírez-Malagón, Neftalí Ochoa-Alejo
      Pages 107-115
    5. Pascal Geerts, André Toussaint, Guy Mergeai, Jean-Pierre Baudoin
      Pages 117-129
    6. Richard Fratini, Maria L. Ruiz
      Pages 131-139
    7. Ajoy Kumar Roy, Devendra Ram Malaviya, Pankaj Kaushal
      Pages 141-151
  4. Cryopreservation of Embryos

    1. Front Matter
      Pages 153-153
    2. Florent Engelmann
      Pages 155-184
    3. Anna De Carlo, Maurizio Lambardi, Elif Aylin Ozudogru
      Pages 185-200
    4. Ana M. Vieitez, M. Carmen San-José, Elena Corredoira
      Pages 201-213
    5. Luis Mroginski, Natalia Dolce, Pedro Sansberro, Claudia Luna, Ana Gonzalez, Hebe Rey
      Pages 215-225
  5. The Use of Zygotic Embryos as Explants for Somatic Embryogenesis and/or Organogenesis

  6. Transformation Using Zygotic Embryos as Explants

    1. Front Matter
      Pages 307-307
    2. Muhammad Tahir, Ejaz A. Waraich, Claudio Stasolla
      Pages 309-326
    3. Bronwyn Frame, Marcy Main, Rosemarie Schick, Kan Wang
      Pages 327-341
  7. Back Matter
    Pages 373-377

About this book


A great fascination for biologists, the study of embryo development provides indispensable information concerning the origins of the various forms and structures that make up an organism, and our ever-increasing knowledge gained through the study of plant embryology promises to lead to the development of numerous useful applications. In Plant Embryo Culture: Methods and Protocols, expert researchers from the field provide a ready source of information for culturing zygotic embryos for different types of studies, both theoretical and practical. The book’s main sections examine a wide range of related topics, including the culture of zygotic embryos for developmental studies, the application of embryo culture techniques focusing on embryo rescue methods, cryopreservation of zygotic embryos, the use of zygotic embryos as explants for somatic embryogenesis and organogenesis, as well as transformation protocols using zygotic embryos as starting material. Written in the highly successful Methods in Molecular Biology™ series format, the detailed chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital notes on troubleshooting and avoiding known pitfalls. Authoritative and convenient, Plant Embryo Culture: Methods and Protocols serves as a key reference that can be used by scientists of all backgrounds to help develop their own customized methods for many different species and for a variety of purposes.


Cryopreservation Embryo development Embryo rescue Explants In vitro culture Isolation Organogenesis Somatic embryogenesis Transformation protocols Zygotic embryos

Editors and affiliations

  • Trevor A. Thorpe
    • 1
  • Edward C. Yeung
    • 2
  1. 1.Dept. Biological SciencesUniversity of CalgaryCalgaryCanada
  2. 2., Department of Biological SciencesUniversity of CalgaryCalgaryCanada

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61737-987-1
  • Online ISBN 978-1-61737-988-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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