Metabolic Profiling

Methods and Protocols

  • Thomas O. Metz

Part of the Methods in Molecular Biology book series (MIMB, volume 708)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Arthur B. Robinson, Noah E. Robinson
    Pages 1-23
  3. Monique Piraud, Séverine Ruet, Sylvie Boyer, Cécile Acquaviva, Pascale Clerc-Renaud, David Cheillan et al.
    Pages 25-53
  4. Giuseppe Lazzarino, Angela Maria Amorini, Valentina Di Pietro, Barbara Tavazzi
    Pages 99-117
  5. Rajan S. Kombu, Henri Brunengraber, Michelle A. Puchowicz
    Pages 147-157
  6. Antonia Garcia, Coral Barbas
    Pages 191-204
  7. Sunil Bajad, Vladimir Shulaev
    Pages 213-228
  8. Heli Nygren, Tuulikki Seppänen-Laakso, Sandra Castillo, Tuulia Hyötyläinen, Matej Orešič
    Pages 247-257
  9. Elizabeth Want, Perrine Masson
    Pages 277-298
  10. Hector C. Keun, Toby J. Athersuch
    Pages 321-334
  11. Timothy M.D. Ebbels, John C. Lindon, Muireann Coen
    Pages 365-388
  12. Back Matter
    Pages 389-391

About this book


At the intersection of metabolite analysis, metabolic fingerprinting, and metabolomics, the study of metabolic profiling has evolved steadily over the course of time as have the methods and technologies involved in its study. In Metabolic Profiling: Methods and Protocols, expert researchers in the field present protocols that are illustrative of the evolution of metabolic profiling from single molecule analysis to global metabolome profiling. Comprised of the most essential techniques, this volume covers topics from inborn errors of metabolism and drug metabolite analysis to nuclear magnetic resonance metabolic profiles. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Metabolic Profiling: Methods and Protocols serves as a resource for both established and new investigators in this vital and ever-developing field.


Drug analysis Global metabolome profiling Inborn errors Metabolic fingerprinting Metabolomics Single molecule analysis Targeted metabolite analysis

Editors and affiliations

  • Thomas O. Metz
    • 1
  1. 1.Fundamental & Computational Sciences DirPacific Northwest National LaboratoryRichlandUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61737-984-0
  • Online ISBN 978-1-61737-985-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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