Adipose-Derived Stem Cells

Methods and Protocols

  • Jeffrey M. Gimble
  • Bruce A. Bunnell

Part of the Methods in Molecular Biology book series (MIMB, volume 702)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Isolation Methods

    1. Front Matter
      Pages 1-1
    2. Christine Gagliardi, Bruce A. Bunnell
      Pages 3-16
    3. Gang Yu, Z. Elizabeth Floyd, Xiying Wu, Yuan-Di C. Halvorsen, Jeffrey M. Gimble
      Pages 17-27
    4. Gang Yu, Xiying Wu, Gail Kilroy, Yuan-Di C. Halvorsen, Jeffrey M. Gimble, Z. Elizabeth Floyd
      Pages 29-36
    5. Martin A. Vidal, Mandi J. Lopez
      Pages 61-75
    6. Kellie J. Williams, Robert A. Godke, Kenneth R. Bondioli
      Pages 77-86
  3. Phenotyping Methods

    1. Front Matter
      Pages 107-107
    2. H. Alan Tucker, Bruce A. Bunnell
      Pages 121-131
    3. Pauline Decaunes, David Estève, Alexia Zakaroff-Girard, Coralie Sengenès, Jean Galitzky, Anne Bouloumié
      Pages 151-161
    4. Peter Scherp, Ginger Ku, Liana Coleman, Indu Kheterpal
      Pages 163-190
  4. Differentiation Methods: In Vitro and In Vivo Applications

    1. Front Matter
      Pages 191-191
    2. Gang Yu, Z. Elizabeth Floyd, Xiying Wu, Teddi Hebert, Yuan-Di C. Halvorsen, Benjamin M. Buehrer et al.
      Pages 193-200
    3. Ji Min Yu, Bruce A. Bunnell, Soo-Kyung Kang
      Pages 219-231
    4. Robert Jan Kroeze, Marlene Knippenberg, Marco N. Helder
      Pages 233-248
    5. Kacey G. Marra, Candace A. Brayfield, J. Peter Rubin
      Pages 261-268
    6. Louis Casteilla, Valérie Planat-Bénard, Stéphanie Dehez, Sandra De Barros, Corinne Barreau, Mireille André
      Pages 269-287
    7. Patrick C. Baer, Martin Brzoska, Helmut Geiger
      Pages 289-298
    8. Jong S. Rim, Karen Strickler, Christian Barnes, Lettie Harkins, Jaroslaw Staszkiewicz, Rachel Power et al.
      Pages 299-316
  5. Bioengineering, Manufacturing, and Clinical Technologies

    1. Front Matter
      Pages 317-317
    2. Jennifer H. Choi, Evangelia Bellas, Gordana Vunjak-Novakovic, David L. Kaplan
      Pages 319-330
    3. Philippe Bourin, Julie-Anne Peyrafitte, Sandrine Fleury-Cappellesso
      Pages 331-343
    4. Patricia A. Zuk
      Pages 345-357
    5. Y. Reneé Lea-Currie, David J. Duffin, Benjamin M. Buehrer
      Pages 359-368
    6. Ram Devireddy, Sreedhar Thirumala
      Pages 369-394
    7. J. Peter Rubin, Kacey G. Marra
      Pages 395-400
    8. Brigitte Wdziekonski, Tala Mohsen-Kanson, Phi Villageois, Christian Dani
      Pages 419-427
    9. Benoît Labbé, Guillaume Marceau-Fortier, Julie Fradette
      Pages 429-441
    10. A. Cagri Uysal, Hiroshi Mizuno
      Pages 443-451
    11. Hiroshi Mizuno, Masaki Nambu
      Pages 453-459
    12. Morikuni Tobita, Hiroshi Mizuno
      Pages 461-470
  6. Back Matter
    Pages 471-473

About this book


During the past decade, a wide range of scientific disciplines have adopted the use of adipose-derived stem/stromal cells (ASCs) as an important tool for research and discovery. In Adipose-Derived Stem Cells: Methods and Protocols, experts from the field, including members of the esteemed International Federation of Adipose Therapeutics and Science (IFATS), provide defined and established protocols in order to further codify the utilization of these powerful and accessible cells. With chapters organized around approaches spanning the discovery, pre-clinical, and clinical processes, much of the emphasis is placed on human ASC, while additional techniques involving small and large animal species are included. As a volume in the highly successful Methods in Molecular Biology™ series, the detailed contributions include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Adipose-Derived Stem Cells: Methods and Protocols serves as a vital reference text for experienced researchers as well as new students on the path to further exploring the incredible potential of ASCs.


Bioengineering Differentiation Human ASCs Isolation Phenotyping Regenerative medicine Stromal cells Tissue engineering

Editors and affiliations

  • Jeffrey M. Gimble
    • 1
  • Bruce A. Bunnell
    • 2
  1. 1., Stem Cell BiologyPennington Biomedical Research CenterBaton RougeUSA
  2. 2.School of Medicine, Department of PharmacologyTulane UniversityNew OrleansUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61737-959-8
  • Online ISBN 978-1-61737-960-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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