Plant Chromosome Engineering

Methods and Protocols

  • James A. Birchler

Part of the Methods in Molecular Biology book series (MIMB, volume 701)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Shyamkumar Barampuram, Zhanyuan J. Zhang
    Pages 1-35
  3. Michal Lieberman-Lazarovich, Avraham A. Levy
    Pages 51-65
  4. Akio Kato, Jonathan C. Lamb, Patrice S. Albert, Tatiana Danilova, Fangpu Han, Zhi Gao et al.
    Pages 67-96
  5. Andreas Houben, Shuhei Nasuda, Takashi R. Endo
    Pages 97-111
  6. Chunhui Xu, Weichang Yu
    Pages 113-130
  7. Robert T. Gaeta, Lakshminarasimhan Krishnaswamy
    Pages 131-146
  8. Yuan-Yeu Yau, Yueju Wang, James G. Thomson, David W. Ow
    Pages 147-166
  9. Feng Zhang, Daniel F. Voytas
    Pages 167-177
  10. Nobuyuki Matoba, Keith R. Davis, Kenneth E. Palmer
    Pages 199-219
  11. Jaroslav Doležel, Marie Kubaláková, Jarmila íhalíková, Pavla Suchánková, Hana Šimková
    Pages 221-238
  12. Ralf G. Kynast, Oscar Riera-Lizarazu
    Pages 259-284
  13. Sivanandan Chudalayandi
    Pages 285-300
  14. Jianbo Zhang, Chuanhe Yu, Lakshminarasimhan Krishnaswamy, Thomas Peterson
    Pages 315-326
  15. Back Matter
    Pages 327-329

About this book


With an increasing human population and a decreasing amount of arable land, creative improvements in agriculture will be a necessity in the coming decades to maintain or improve the standard of living. In Plant Chromosome Engineering: Methods and Protocols, expert researchers present techniques for the modification of crops and other plant species in order to achieve the goal of developing the much needed novel approaches to the production of food, feed, fuel, fiber, and pharmaceuticals. This volume examines vital topics such as transformation procedures, chromosome painting, production of engineered minichromosomes, gene targeting and mutagenesis, site specific integration, gene silencing, protein expression, chromosome sorting and analysis, protocols for generating chromosomal rearrangements, enhancer trapping, and means of studying chromosomes in vivo. As a part of the highly successful Methods in Molecular Biology™ series, the methodological chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and professional tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant Chromosome Engineering: Methods and Protocols highlights the spectrum of tools currently available for modifying plant genomes and chromosomes and provides the foundation for crucial future developments.


Agricultural improvement Chromosomal rearrangements Engineered minichromosomes Gene targeting and silencing Modified crops Mutagenesis Pharmaceuticals Plant species Protein expression Transformation procedures

Editors and affiliations

  • James A. Birchler
    • 1
  1. 1., Division of Biological SciencesUniversity of MissouriColumbiaUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61737-956-7
  • Online ISBN 978-1-61737-957-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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