Flow Cytometry Protocols

  • Teresa S. Hawley
  • Robert G. Hawley

Part of the Methods in Molecular Biology book series (MIMB, volume 699)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Alice L. Givan
    Pages 1-29
  3. C. Bruce Bagwell
    Pages 31-51
  4. Lili Wang, Adolfas K. Gaigalas, Ming Yan
    Pages 53-65
  5. Tione Buranda, Yang Wu, Larry A. Sklar
    Pages 67-84
  6. Henri C. van der Heyde, Irene Gramaglia
    Pages 85-95
  7. Shane Bruckner, Ling Wang, Ruiling Yuan, Perry Haaland, Amitabh Gaur
    Pages 97-118
  8. Joseph D. Tario Jr., Katharine A. Muirhead, Dalin Pan, Mark E. Munson, Paul K. Wallace
    Pages 119-164
  9. Patricia Lovelace, Holden T. Maecker
    Pages 165-178
  10. Peter O. Krutzik, Angelica Trejo, Kenneth R. Schulz, Garry P. Nolan
    Pages 179-202
  11. William G. Telford, Akira Komoriya, Beverly Z. Packard, C. Bruce Bagwell
    Pages 203-227
  12. James W. Jacobberger, R. Michael Sramkoski, Tammy Stefan
    Pages 229-249
  13. Cornelis J. H. Pronk, David Bryder
    Pages 275-293
  14. Katherine R. Calvo, Catharine S. McCoy, Maryalice Stetler-Stevenson
    Pages 295-316
  15. Maurice R. G. O’Gorman, Joshua Zollett, Nicolas Bensen
    Pages 317-335
  16. Henri C. van der Heyde, Irene Gramaglia, Valéry Combes, Thaddeus C. George, Georges E. Grau
    Pages 337-354
  17. Rita L. Strack, Robert J. Keenan, Benjamin S. Glick
    Pages 355-370
  18. György Vereb, Péter Nagy, János Szöllo˝si
    Pages 371-392
  19. M. Raza Zaidi, Chi-Ping Day, Glenn Merlino
    Pages 393-405
  20. Irene Riz, Teresa S. Hawley, Robert G. Hawley
    Pages 431-447
  21. Stephen P. Perfetto, David R. Ambrozak, Richard Nguyen, Mario Roederer, Richard A. Koup, Kevin L. Holmes
    Pages 449-469
  22. Howard M. Shapiro
    Pages 471-482
  23. Teresa S. Hawley, Robert G. Hawley
    Pages E1-E2
  24. Back Matter
    Pages 483-485

About this book


With rapid improvements in instrumentation, lasers, fluorophores, and data analysis software, flow cytometry is riding the crest of unprecedented, innovative advances. This thoroughly revised and up-to-date third edition of Flow Cytometry Protocols highlights the expanding contribution of flow cytometry to basic biological research and diagnostic medicine. Written by leading experts in the field, the book presents cutting-edge topics such as polychromatic, quantitative, and high throughput flow cytometry, novel multiparametric data analysis which breaks the dimensionality barrier, standard practice and safety measures for aerosol-generating cell sorting, conventional and imaging flow cytometry as well as minimalist imaging cytometry. As a volume in the highly successful Methods in Molecular Biology™ series, chapters contain brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and extensive notes on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Flow Cytometry Protocols, Third Edition presents established as well as new flow cytometric methodologies in order to introduce beginning users to basic applications while opening new avenues of innovation for seasoned users.


Cell sorting Combinatorial antibody profiling Diagnostic medicine Fluorescent proteins Imaging Immunophenotyping Phospho-flow Polychromatic characterizations Quantum dots

Editors and affiliations

  • Teresa S. Hawley
    • 1
  • Robert G. Hawley
    • 2
  1. 1.Medical Center, Flow Cytometry Core FacilityGeorge Washington UniversityWashingtonUSA
  2. 2.Medical Center, Dept. Anatomy & Regenerative BiologyGeorge Washington UniversityWashingtonUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61737-949-9
  • Online ISBN 978-1-61737-950-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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