Mesenchymal Stem Cell Assays and Applications

  • Mohan Vemuri
  • Lucas G. Chase
  • Mahendra S. Rao

Part of the Methods in Molecular Biology book series (MIMB, volume 698)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Introduction

    1. Front Matter
      Pages 1-1
    2. Mohan C. Vemuri, Lucas G. Chase, Mahendra S. Rao
      Pages 3-8
  3. Isolation and Expansion of MSCs from Various Sources

    1. Front Matter
      Pages 9-9
    2. Patrick Horn, Simone Bork, Wolfgang Wagner
      Pages 23-35
    3. Vladimir Zachar, Jeppe Grøndahl Rasmussen, Trine Fink
      Pages 37-49
    4. Justin D. Klein, Dario O. Fauza
      Pages 75-88
    5. Nina Ilic, Gary Brooke, Patricia Murray, Sarah Barlow, Tony Rossetti, Rebecca Pelekanos et al.
      Pages 89-106
    6. Stan Gronthos, Agnieszka Arthur, P. Mark Bartold, Songtao Shi
      Pages 107-121
    7. Karen L. Seeberger, Alana Eshpeter, Gregory S. Korbutt
      Pages 123-140
    8. Ruenn Chai Lai, Andre Choo, Sai Kiang Lim
      Pages 141-150
    9. Mandana Haack-Sørensen, Jens Kastrup
      Pages 161-174
    10. Hicham Majd, Thomas M. Quinn, Pierre-Jean Wipff, Boris Hinz
      Pages 175-188
    11. Francisco dos Santos, Pedro Z. Andrade, Gemma Eibes, Cláudia Lobato da Silva, Joaquim M. S. Cabral
      Pages 189-198
  4. MSC Lineage Differentiation and Analysis

    1. Front Matter
      Pages 199-199
    2. Deepak M. Gupta, Nicholas J. Panetta, Michael T. Longaker
      Pages 201-214
    3. Ulf Krause, Anja Seckinger, Carl A. Gregory
      Pages 215-230
    4. Warren L. Grayson, Sarindr Bhumiratana, Christopher Cannizzaro, Gordana Vunjak-Novakovic
      Pages 231-241
    5. Trine Fink, Vladimir Zachar
      Pages 243-251
    6. Luis A. Solchaga, Kitsie J. Penick, Jean F. Welter
      Pages 253-278
    7. Katarzyna A. Trzaska, Pranela Rameshwar
      Pages 295-303
    8. Sarah Snykers, Joery De Kock, Vanhaecke Tamara, Vera Rogiers
      Pages 305-314
    9. Bruno Christ, Sandra Brückner, Peggy Stock
      Pages 315-330
    10. Lindolfo da Silva Meirelles, Dimas Tadeu Covas
      Pages 331-350
  5. MSC Phenotypic Characterization and Extended Applications

    1. Front Matter
      Pages 351-351
    2. Monique Provansal, Christian Jorgensen, Sylvain Lehmann, Stéphane Roche
      Pages 431-442
    3. Guo Li, Chu-yan Chan, Hua Wang, Hsiang-fu Kung
      Pages 443-457
    4. Courtenay Hart, Lucas G. Chase, Mahbod Hajivandi, Brian Agnew
      Pages 459-484
    5. Michael O’Grady, Robert H. Batchelor, Kelly Scheyhing, Christopher W. Kemp, George T. Hanson, Uma Lakshmipathy
      Pages 485-504
    6. Debanjan Sarkar, Weian Zhao, Ashish Gupta, Wei Li Loh, Rohit Karnik, Jeffrey M. Karp
      Pages 505-523
  6. Back Matter
    Pages 525-534

About this book


Mesenchymal Stem Cells have seen an unprecedented level of interest in the last decade, primarily due to their relative ease of isolation, the large numbers of cells present in the adult, and the ability to propagate these cells in culture. In Mesenchymal Stem Cell Assays and Applications, expert researchers from across the globe explore the latest techniques to propagate, characterize, and engineer this special cell type. Chapters outline a set of protocols and assays used by leading investigators in the field, providing standards that can be applied by all researchers to the population of cells used in their experiments. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Ground-breaking and current, Mesenchymal Stem Cell Assays and Applications is a necessary handbook for all researchers working with this ambiguous population of cells.


Human Cord Blood-Derived MSC Human Multipotent Stromal Cells MSC Marrow derived stromal cells

Editors and affiliations

  • Mohan Vemuri
    • 1
  • Lucas G. Chase
    • 2
  • Mahendra S. Rao
    • 3
  1. 1., Primary and Stem Cell SystemsLife TechnologiesFrederickUSA
  2. 2.Cellular Dynamics International, Inc.MadisonUSA
  3. 3.Life TechnologiesFrederickUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60761-998-7
  • Online ISBN 978-1-60761-999-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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