3D Cell Culture

Methods and Protocols

  • John W. Haycock

Part of the Methods in Molecular Biology book series (MIMB, volume 695)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Eleonora Carletti, Antonella Motta, Claudio Migliaresi
    Pages 17-39
  3. Sarah H. Cartmell, Sarah Rathbone, Gemma Jones, L. Araida Hidalgo-Bastida
    Pages 61-76
  4. David A. Lee, June Brand, Donald Salter, Oto-Ola Akanji, Tina T. Chowdhury
    Pages 77-97
  5. Teresa Coughlan, Aileen Crawford, Paul Hatton, Michael Barker
    Pages 99-114
  6. Paul J. Kingham, Cristina Mantovani, Giorgio Terenghi
    Pages 115-127
  7. Sheila MacNeil, Joanna Shepherd, Louise Smith
    Pages 129-153
  8. Celia Murray-Dunning, Sally L. McArthur, Tao Sun, Rob McKean, Anthony J. Ryan, John W. Haycock
    Pages 155-166
  9. Rui C. Pereira, Chiara Gentili, Ranieri Cancedda, Helena S. Azevedo, Rui L. Reis
    Pages 167-181
  10. James B. Phillips, Robert Brown
    Pages 183-196
  11. Ronald E. Unger, Sven Halstenberg, Anne Sartoris, C. James Kirkpatrick
    Pages 229-241
  12. Noha M. Zaki, Nicola Tirelli
    Pages 243-259
  13. Lee Buttery, Robert Bielby, Daniel Howard, Kevin Shakesheff
    Pages 281-308
  14. Eleanor Knight, Bridgid Murray, Ross Carnachan, Stefan Przyborski
    Pages 323-340
  15. Back Matter
    Pages 341-343

About this book


Developed for a range of tissues where the culture environment takes into account the spatial organization of the cells therein, 3D cell culture models serve to bridge the gap between in vivo studies at one extreme with that of simple cell monolayers at the other. In 3D Cell Culture: Methods and Protocols, international experts describe a number of basic and applied methodologies taken from a breadth of scientific and engineering disciplines, many of which deal with direct applications of 3D culture models, most notably in the formation of tissues for clinical purpose. Beginning with an overview of the biological and materials scaffold requirements for successfully creating 3D models, the book delves into topics such as general scaffold design and fabrication techniques, models for bone, skin, cartilage, nerve, bladder, and hair follicles, and chapters on bioreactor design, imaging, and stem cells. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective subjects, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, 3D Cell Culture: Methods and Protocols serves as a basic manual for laboratory-based scientists who not only need to have a comprehensive range of techniques contained within a single text but also require techniques described using a standard, convenient format.


Bioreactor design Growth environment Regenerative medicine Scaffolds Stem cell technology Three dimensional models Tissue engineering

Editors and affiliations

  • John W. Haycock
    • 1
  1. 1.Kroto Research Institute, Department of Engineering MaterialsUniversity of SheffieldSheffieldUnited Kingdom

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60761-983-3
  • Online ISBN 978-1-60761-984-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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