Quorum Sensing

Methods and Protocols

  • Kendra P. Rumbaugh

Part of the Methods in Molecular Biology book series (MIMB, volume 692)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Detection, Isolation, and Characterization of Quorum Sensing Compounds

    1. Front Matter
      Pages 1-1
    2. Weihua Chu, Dhiraj A. Vattem, Vatsala Maitin, Mary B. Barnes, Robert J. C. McLean
      Pages 3-19
    3. Stephen P. Diggle, Matthew P. Fletcher, Miguel Cámara, Paul Williams
      Pages 21-30
    4. Kirthiram K. Sivakumar, Palmy R. Jesudhasan, Suresh D. Pillai
      Pages 71-82
    5. Peter Edmonson, Desmond Stubbs, William Hunt
      Pages 83-100
    6. Colin A. Lowery, Gunnar F. Kaufmann, Kim D. Janda
      Pages 101-111
  3. Determining the Function of Autoinducers In vivo

    1. Front Matter
      Pages 131-131
    2. Vladimir V. Kravchenko, Richard J. Ulevitch, Gunnar F. Kaufmann
      Pages 133-145
    3. Louise Dahl Christensen, Maria van Gennip, Tim Holm Jakobsen, Michael Givskov, Thomas Bjarnsholt
      Pages 147-157
    4. Mair E. A. Churchill, Hiruy M. Sibhatu, Charis L. Uhlson
      Pages 159-171
    5. Gregory C. Palmer, Jeffrey W. Schertzer, Lauren Mashburn-Warren, Marvin Whiteley
      Pages 207-217
    6. Aurélie Deveau, Deborah A. Hogan
      Pages 219-233
    7. Wilfried Weber, Martin Fussenegger
      Pages 235-249
  4. Quorum Sensing Disruption Strategies

    1. Front Matter
      Pages 251-251
    2. Tim Holm Jakobsen, Maria van Gennip, Louise Dahl Christensen, Thomas Bjarnsholt, Michael Givskov
      Pages 253-263
    3. Jun Igarashi, Hiroaki Suga
      Pages 265-274
    4. Gunnar F. Kaufmann, Junguk Park, Alexander V. Mayorov, Diane M. Kubitz, Kim D. Janda
      Pages 299-311
  5. Back Matter
    Pages 313-315

About this book


Since its early days in the 1990s, the Quorum Sensing (QS) field has grown from a few dozen laboratories, investigating the pathways, proteins, and chemicals that facilitate signaling in bacteria, to hundreds of groups that have integrated evolutionary biology, computer science, mathematics, engineering, and metagenomics to create an ever-expanding and dynamic field. In Quorum Sensing: Methods and Protocols, expert researchers provide an in-depth set of diverse protocols that span this broad area of study. Broken into three detailed sections, the volume covers the detection, isolation, and characterization of the QS signals made by both Gram- and Gram+ bacteria, determination of the function of QS signals in vivo, and the development of QS disruption strategies. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known experimental pitfalls. Comprehensive and cutting-edge, Quorum Sensing: Methods and Protocols serves as an invaluable collection of easily accessible techniques for scientists seeking to further our knowledge about bacterial communication and its relation to humanity.


Antibiotic resistant infections Bacteria Biofilms Cell-to-cell signaling Communication systems Gram- and Gram+ QS disruption QS signals

Editors and affiliations

  • Kendra P. Rumbaugh
    • 1
  1. 1.Health Sciences Center, Department of SurgeryTexas Tech UniversityLubbockUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60761-970-3
  • Online ISBN 978-1-60761-971-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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