Enzyme Stabilization and Immobilization

Methods and Protocols

  • Shelley D. Minteer

Part of the Methods in Molecular Biology book series (MIMB, volume 679)

Table of contents

  1. Front Matter
    Pages i-x
  2. Michael J. Moehlenbrock, Shelley D. Minteer
    Pages 1-7
  3. Andreas Buthe, Songtao Wu, Ping Wang
    Pages 37-48
  4. Allan E. David, Arthur J. Yang, Nam Sun Wang
    Pages 49-66
  5. Keith J. Stine, Kenise Jefferson, Olga V. Shulga
    Pages 67-83
  6. Glenn R. Johnson, Heather R. Luckarift
    Pages 85-97
  7. Zorica D. Knežević-Jugović, Dejan I. Bezbradica, Dušan Ž. Mijin, Mirjana G. Antov
    Pages 99-111
  8. Sabina Besic, Shelley D. Minteer
    Pages 113-131
  9. Munishwar N. Gupta, Smita Raghava
    Pages 133-145
  10. Nenad B. Milosavić, Radivoje M. Prodanović
    Pages 155-163
  11. Alexey A. Vertegel, Vladimir Reukov, Victor Maximov
    Pages 165-182
  12. Patrick A. Johnson, Hee Joon Park, Ashley J. Driscoll
    Pages 183-191
  13. Amanda S. Harper-Leatherman, Jean Marie Wallace, Debra R. Rolison
    Pages 193-205
  14. Michael J. Cooney
    Pages 207-225
  15. Back Matter
    Pages 227-229

About this book


In the past decade, researchers have made tremendous progress in the field of enzyme stabilization, opening up new opportunities for enzymes in molecular biology and for industrial applications. In Enzyme Stabilization and Immobilization: Methods and Protocols, expert researchers explore the latest developments through detailed laboratory protocols, which address many different theories and techniques in enzyme stabilization. Chapters outline protocols for enzyme stabilization in solutions, including: liposome formation, micelle introduction, crosslinking, and additives. Secondly, the book contain protocols for enzyme stabilization via enzyme immobilization, such as sol-gel encapsulation, polymer encapsulation, and single enzyme nanoparticle formation. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and current, Enzyme Stabilization and Immobilization: Methods and Protocols is an essential handbook for all molecular biologists, biochemists, and biomedical and biochemical engineers.


Enzyme encapsulation Enzyme immobilization Gel entrapment Thermal stability pH tolerance

Editors and affiliations

  • Shelley D. Minteer
    • 1
  1. 1.Department of ChemistrySt. Louis UniversitySt. LouisUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60761-894-2
  • Online ISBN 978-1-60761-895-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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