Confocal Microscopy

Methods and Protocols

  • Stephen W. Paddock

Part of the Methods in Molecular Biology book series (MIMB, volume 1075)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Kristin L. Hazelwood, Christopher S. Murphy, Zachary C. Cunningham, Matthew J. Parry-Hill, Richard L. Ludlow, Ericka B. Ramko et al.
    Pages 49-96
  3. Timo Zimmermann, Joanne Marrison, Karen Hogg, Peter O’Toole
    Pages 129-148
  4. George McNamara, Anna Yanai, Vazgen Khankaldyyan, Walter E. Laug, Jeff Boden, Keith Webster et al.
    Pages 149-175
  5. Craig R. Brunetti
    Pages 177-184
  6. Robert L. Price, Stephen T. Haley, Tara Bullard, Jeffrey Davis, Thomas K. Borg, Louis Terracio
    Pages 185-199
  7. Angela V. Klaus, Valerie Schawaroch, Kevin J. Frischmann
    Pages 213-225
  8. Paulo J. Oliveira, Edward L. Perkins, Jon Holy
    Pages 227-241
  9. Barbara Fasulo, William Sullivan
    Pages 243-255
  10. Robert L. Conhaim, Dennis M. Heisey, Glen E. Leverson
    Pages 297-304
  11. Luise A. Cullen-McEwen, Richard J. Young, Gabriel Fricout, Dominique Jeulin, Ian S. Harper, Frank Costantini et al.
    Pages 305-320
  12. Robert M. Zucker
    Pages 321-374
  13. Stephen W. Paddock
    Pages E1-E3
  14. Back Matter
    Pages 375-381

About this book


Confocal Microscopy: Methods and Protocols, Second Edition takes the researcher from the bench top through the imaging process, to the page. Protocols for the preparation of tissues from many model organisms including worms, flies and mice have been included as well as chapters on confocal imaging of living cells, three dimensional analysis, and the measurement and presentation of confocal images for publication. Emphasis has been placed on the laser scanning confocal microscope since this is still the instrument used for most routine applications. The current generation of modern confocal instruments produces optical sections of cells and tissues that are free of out-of-focus fluorescence with reduced chances of artifacts from the techniques of specimen preparation. This allows the imaging of living specimens and measurements of physiological events within cells. Confocal microscopy has become essential in many fields of contemporary biomedical research where a light microscope is required for imaging fluorescently labeled cells and tissues, especially cell biology, developmental biology, neurobiology, and pathology. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and easily accessible, Confocal Microscopy: Methods and Protocols, Second Edition is aimed primarily, but not exclusively, at the novice user with pointers to more advanced techniques. 


confocal imaging confocal microscope confocal microscopy confocal system fluorescence microscopy

Editors and affiliations

  • Stephen W. Paddock
    • 1
  1. 1.University of Wisconsin Dept. of Molecular BiologyMadisonUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2014
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-351-0
  • Online ISBN 978-1-60761-847-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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