Oral Biology

1. Cells and Tissues

   1. A Method to Isolate, Purify, and Characterize Human Periodontal Ligament Stem Cells

      • Krzysztof Mrozik, Stan Gronthos, Songtao Shi, P. Mark Bartold

      Pages 269-284

   2. Preclinical Methods for the Evaluation of Periodontal Regeneration In Vivo

      • Yang-Jo Seol, Gaia Pellegrini, Lea M. Franco, Po-Chun Chang, Chan Ho Park, William V. Giannobile

      Pages 285-307

   3. Proteomic Analysis of Dental Tissue Microsamples

      • Jonathan E. Mangum, Jew C. Kon, Michael J. Hubbard

      Pages 309-325

   4. Immunological Techniques: ELISA, Flow Cytometry, and Immunohistochemistry

      • Pauline J. Ford

      Pages 327-343

   5. Analysis of Immune Responses to Purified Recombinant Antigens of Periodontal Pathogens

      • Koichi Tabeta, Kazuhisa Yamazaki

      Pages 345-357

   6. Single-Strand Conformation Polymorphism Analysis for the Diagnosis of T-Cell Clonality in Periodontal Disease

      • Kazuhisa Yamazaki, Harue Ito

      Pages 359-372

   7. Real-Time PCR Focused-Gene Array Profiling of Gingival and Periodontal Ligament Fibroblasts

      • Patty Chou, Trudy J. Milne

      Pages 373-383

   8. The Use of Gene Arrays in Deciphering the Pathobiology of Periodontal Diseases

      • Moritz Kebschull, Panos N. Papapanou

      Pages 385-393

   9. Bioinformatics Techniques in Microarray Research: Applied Microarray Data Analysis Using R and SAS Software

      • Ryan T. Demmer, Paul Pavlidis, Panos N. Papapanou

      Pages 395-417

2. Back Matter

   Pages 419-422

   PDF

It is generally recognized that the knowledge and research base that underpins dentistry lies in the biological and physical sciences. In this context, the major advances in these sciences over the past two decades have come through the application of molecular bi- ogy and nanotechnology. These advances are currently impacting on the diagnosis and treatment of a wide range of human diseases and it is essential that dental research, education, and practice keep pace with this rapidly advancing ?eld. As pointed out by Ford et al. (1): The de?nition of disease is also changing. Previously, disease was understood to be the presence of symptoms or of a particular phenotype. With increasing knowledge of the genetic basis of many diseases, this de?nition is changing to become the presence of a genotype conferring a pre-disposition to clinical symptoms or phenotype (Ford et al. (1)). This changing de?nition of disease means that today’s undergraduate or graduate student in dentistry (and its related ?elds) must be in a position not only to acquire new knowledge in the future but also to be able to evaluate the information and apply it in a clinically relevant setting. This naturally positions oral biology as an integral part of any dentally related professional’s repertoire of knowledge. There are as many topics in oral biology as there are the number of sites and micro- vironments within the oral cavity.

• Biofilm
• Bioinformatic analysis
• Dental diseases
• Dentistry
• Microarray
• Nanotechnology
• Oral cell biology
• Oral microbiota
• Saliva
• bioinformatics
• genome
• microbiology
• tissue

From the reviews:

“This book comprehensively reviews a wide range of techniques and protocols from diverse areas of oral biology, encompassing current research on microbes, host tissues and saliva. The book is obviously aimed at active researchers in the various fields, but because the research topics are so diverse, I would recommend purchase by institutes and departments … .” (Adam Roberts, Microbiology Today, May, 2011)

• Faculty of Dentistry, Department of Oral Sciences, University of Otago, Dunedin, New Zealand

  Gregory J. Seymour, Mary P. Cullinan

• Faculty of Dentistry, Sir John Walsh Research Institute, University of Otago, Dunedin, New Zealand

  Nicholas C.K. Heng

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