Plant Reverse Genetics

Methods and Protocols

  • Andy Pereira

Part of the Methods in Molecular Biology book series (MIMB, volume 678)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Shrinivasrao P. Mane, Thero Modise, Bruno W. Sobral
    Pages 1-11
  3. Saroj K. Mohapatra, Arjun Krishnan
    Pages 27-43
  4. Madana M. R. Ambavaram, Andy Pereira
    Pages 45-54
  5. Andrew Hayward, Meenu Padmanabhan, S. P. Dinesh-Kumar
    Pages 55-63
  6. Zarir Vaghchhipawala, Clemencia M. Rojas, Muthappa Senthil-Kumar, Kirankumar S. Mysore
    Pages 65-76
  7. Mieko Higuchi, Youichi Kondou, Takanari Ichikawa, Minami Matsui
    Pages 77-89
  8. Nayelli Marsch-Martínez, Andy Pereira
    Pages 91-105
  9. Michael A. Ayliffe, Anthony J. Pryor
    Pages 107-128
  10. Chyr-Guan Chern, Ming-Jen Fan, Sheng-Chung Huang, Su-May Yu, Fu-Jin Wei, Cheng-Chieh Wu et al.
    Pages 129-138
  11. Narayana M. Upadhyaya, Qian-Hao Zhu, Ramesh S. Bhat
    Pages 147-177
  12. Xiaofei Cheng, Jiangqi Wen, Million Tadege, Pascal Ratet, Kirankumar S. Mysore
    Pages 179-190
  13. Pieter B. F. Ouwerkerk, Annemarie H. Meijer
    Pages 211-227
  14. Joel L. Shuman, Diego F. Cortes, Jenny M. Armenta, Revonda M. Pokrzywa, Pedro Mendes, Vladimir Shulaev
    Pages 229-246
  15. Back Matter
    Pages 277-282

About this book


After the generation of genome sequence data from a wide variety of plants, databases are filled with sequence information of genes with no known biological function, and while bioinformatics tools can help analyze genome sequences and predict gene structures, experimental approaches to discover gene functions need to be widely implemented. In Plant Reverse Genetics: Methods and Protocols, leading researchers in the field describe cutting-edge methods, both high-throughput and genome-wide, involving the models Arabidopsis and rice as well as several other plants to provide comparative functional genomics information. With chapters on the analysis of high-throughput genome sequence data, the identification of non-coding RNA from sequence information, the comprehensive analysis of gene expression by microarrays, and metabolomic analysis, the thorough methods of the book are fully supported by scripts to aid their computational use. Written in the highly successful Methods in Molecular Biology™ series format, the chapters contain introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and essential, Plant Reverse Genetics: Methods and Protocols is an ideal guide for researchers seeking an understanding of how the complex web of plant genes work together in a systems biology view.


Arabidopsis and rice Developmental mutant phenotypes Functional genomics Genome sequencing Metabolomic analysis Microarrays Non-coding RNA Systems biology

Editors and affiliations

  • Andy Pereira
    • 1
  1. 1., Virginia Bioinformatics InstituteVirginia TechBlacksburgUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60761-681-8
  • Online ISBN 978-1-60761-682-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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