In Vitro Mutagenesis Protocols

Third Edition

  • Jeff Braman

Part of the Methods in Molecular Biology book series (MIMB, volume 634)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Mutagenesis in Various Microbial Backgrounds

    1. Front Matter
      Pages 1-1
    2. Thomas A. Ficht, Jianwu Pei, Melissa Kahl-McDonagh
      Pages 15-35
    3. Duncan J. H. Gaskin, Arnoud H. M. van Vliet
      Pages 37-52
    4. Zhen Zhang, Ying Huang, Hua Zhu
      Pages 75-86
  3. PCR Mutagenesis

  4. Reviews

  5. Protein Evolution Mutagenesis

    1. Front Matter
      Pages 216-216
    2. Christian Heinis, Kai Johnsson
      Pages 217-232
    3. Ai-Sheng Xiong, Quan-Hong Yao, Ri-He Peng, Zong-Ming Cheng
      Pages 239-256
    4. Hayato Yanagida, Tomoaki Matsuura, Tetsuya Yomo
      Pages 257-267
  6. Protein Structure and Function Mutagenesis

  7. Random Mutagenesis

    1. Front Matter
      Pages 372-372
    2. Philippe Mondon, David Grand, Nathalie Souyris, Stéphane Emond, Khalil Bouayadi, Hakim Kharrat
      Pages 373-386
    3. Robert A. Smith
      Pages 387-397
    4. Vivian Q. Zhang, Holly H. Hogrefe
      Pages 399-407
  8. Mutator Bacterial Strain Mutagenesis

    1. Front Matter
      Pages 410-410
    2. Ghazala Muteeb, Ranjan Sen
      Pages 411-419
    3. B. Karsten Tischer, Gregory A. Smith, Nikolaus Osterrieder
      Pages 421-430
  9. Back Matter
    Pages 431-442

About this book


In the post-genomic era, in vitro mutagenesis has emerged as a critically important tool for establishing the functions of components of the proteome. The third edition of In Vitro Mutagenesis Protocols represents a practical toolbox containing protocols vital to advancing our understanding of the connection between nucleotide sequence and sequence function. Fully updated from the previous editions, this volume contains a variety of specialty tools successfully employed to unravel the intricacies of protein-protein interaction, protein structure-function, protein regulation of biological processes, and protein activity, as well as a novel section on mutagenesis methods for unique microbes as a guide to the generalization of mutagenesis strategies for a host of microbial systems. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls.

Authoritative and up-to-date, In Vitro Mutagenesis Protocols, Third Edition offers today's researchers a valuable compendium of reliable and powerful techniques with which to illuminate the proteome and its rich web of biological implications.


BAC Chromosom DNA Escherichia coli Microbial systems Nucleotide sequences PCR Protein regulation Protein structure-function Protein-protein interaction Regulation Sequence function molecular biology protein structure transcription

Editors and affiliations

  • Jeff Braman
    • 1
  1. 1.StratageneLa JollaUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2010
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60761-651-1
  • Online ISBN 978-1-60761-652-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site