Lentivirus Gene Engineering Protocols

Second Edition

  • Maurizio Federico

Part of the Methods in Molecular Biology book series (MIMB, volume 614)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Overview

  3. Advances in Lentiviral Vector Technology

    1. Front Matter
      Pages 38-38
    2. María Mercedes Segura, Alain Garnier, Yves Durocher, Sven Ansorge, Amine Kamen
      Pages 39-52
    3. Daniela Bischof, Kenneth Cornetta
      Pages 53-68
    4. David Markusic, Jurgen Seppen
      Pages 69-76
    5. Ali Ramezani, Robert G. Hawley
      Pages 77-100
    6. Zuleika Michelini, Donatella Negri, Andrea Cara
      Pages 101-110
    7. Claudia Muratori, Roberta Bona, Maurizio Federico
      Pages 111-124
  4. New Lentiviral Vector Applications

    1. Front Matter
      Pages 126-126
    2. Mark S. K. Chong, Jerry Chan
      Pages 135-147
    3. Valeria Coppola, Cesare Galli, Maria Musumeci, Désirée Bonci
      Pages 149-160
  5. The Short RNA Technologies Applied by Lentiviral Vectors

    1. Front Matter
      Pages 174-174
    2. Michaela Scherr, Letizia Venturini, Matthias Eder
      Pages 175-185
    3. Hidetoshi Sumimoto, Yutaka Kawakami
      Pages 187-199
    4. Olivier ter Brake, Jan-Tinus Westerink, Ben Berkhout
      Pages 201-213
    5. Kevin A. Peng, Eliezer Masliah
      Pages 215-224
  6. Back Matter
    Pages 225-228

About this book


Since the publication of the first edition, lentivirus vector-based technologies, through in vitro and in vivo gene transfer in eukaryotic animal cells, continue to offer the most promising opportunities for curing genetic disorders, as well as cancer and infectious diseases. Lentivirus Gene Engineering Protocols, Second Edition reflects the spectacular progress made in the field with a set of cutting-edge methods contributed by highly respected scientists. Beginning with a thorough overview of the most recent lentivirus developments, the book continues with detailed protocols including sections on the advances in lentiviral vector technology, new lentiviral vector applications, involving transgenic human embryonic stem cells and fetal gene therapy among other topics, as well as the invaluable breakthroughs in LV-mediated expression of microRNAs. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes sections, highlighting tips on troubleshooting and avoiding known pitfalls.

Authoritative and timely, Lentivirus Gene Engineering Protocols, Second Edition covers the most relevant issues and techniques of LV-based gene engineering, thus representing a complete theoretical and practical guide for scientists still unfamiliar with LV technologies and those who simply wish to know more about this vital area of study.


RNA Virus-like particle gene transfer genes miRNAs molecular biology vaccine virus

Editors and affiliations

  • Maurizio Federico
    • 1
  1. 1.Div. Patogenesi dei RetrovirusCentro Nazionale AIDSRomaItaly

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2010
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60761-532-3
  • Online ISBN 978-1-60761-533-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site