T-Cell Trafficking

Methods and Protocols

  • Federica M. Marelli-Berg
  • Sussan Nourshargh

Part of the Methods in Molecular Biology book series (MIMB, volume 616)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Introductory Review

    1. Front Matter
      Pages 1-1
    2. Alf Hamann
      Pages 3-13
  3. Migration of T Cells In Vitro

    1. Front Matter
      Pages 15-15
    2. Olga Barreiro, Francisco Sánchez-Madrid, María Yáñez-Mó
      Pages 17-30
    3. Charlotte Lawson, Marlene Rose, Sabine Wolf
      Pages 31-47
    4. Helen M. McGettrick, Chris D. Buckley, G. Ed Rainger, Gerard B. Nash
      Pages 49-68
    5. Eva Cernuda-Morollón, Severine Gharbi, Jaime Millán
      Pages 69-82
    6. Marouan Zarrouk, David Killock, Aleksandar Ivetic
      Pages 83-95
    7. Leo M. Carlin, Konstantina Makrogianneli, Melanie Keppler, Gilbert O. Fruhwirth, Tony Ng
      Pages 97-113
    8. Fabrizio Vianello, Elda Righi, Mark C. Poznansky
      Pages 115-124
    9. Anna Korniejewska, Malcolm Watson, Stephen Ward
      Pages 125-147
    10. Petros X. E. Mouratidis, Andrew J. T. George
      Pages 149-158
  4. Migration of T Cells In Vivo

    1. Front Matter
      Pages 159-159
    2. Henrique Veiga-Fernandes, Katie Foster, Amisha Patel, Mark Coles, Dimitris Kioussis
      Pages 161-179
    3. Owain R. Millington, James M. Brewer, Paul Garside, Pasquale Maffia
      Pages 193-206
    4. D. Zhu, S. Jarmin, A. Ribeiro, F. Prin, S.Q. Xie, K. Sullivan et al.
      Pages 207-228
  5. Monitoring T-Cell Migration in Human Diseases

    1. Front Matter
      Pages 229-229
    2. Patricia F. Lalor, Stuart M. Curbishley, David H. Adams
      Pages 231-252
    3. Claire L. Gorman, Claudia Monaco, Enrico Ammiratti, Anna-Chiara Vermi, Federica M. Marelli-Berg, Andrew P. Cope
      Pages 253-266
  6. Back Matter
    Pages 281-290

About this book


In the last decade, a large number of major discoveries have shed light on the molecular mechanisms of lymphocyte migration and the anatomy of immune responses. In T-Cell Trafficking: Methods and Protocols, expert researchers explore how the development of novel and cutting-edge techniques, particularly in the field of real-time imaging and genetic manipulation, have led to an increased understanding of lymphocyte trafficking. Written by internationally recognized experts in their respective fields, chapters provide state-of-the-art protocols to study lymphocyte migration and T-cell: endothelial cell interactions in vitro, address various approaches used for direct visualization of the development of the lymphoid system, lymphocyte recirculation, and effector responses in experimental models in vivo, and explore lymphocyte migration and inflammation in the human system. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Innovative and highly practical, T-Cell Trafficking: Methods and Protocols is an essential manual for newcomers in this ever-expanding and exciting area of research, as well as a valuable addition to more specialized laboratories.


Antigen Fugetaxis Imaging techniques Laboratory Lymphocyte migration Migratory mechanisms Multiphoton microscopy T cell-endothelial cell interactions molecular biology molecular mechanisms

Editors and affiliations

  • Federica M. Marelli-Berg
    • 1
  • Sussan Nourshargh
    • 2
  1. 1.Hammersmith Hospital, Dept. ImmunologyImperial College LondonLondonUnited Kingdom
  2. 2.School of Medicine and Dentistry, William Harvey Research Inst.Queen Mary, University of LondonLondonUnited Kingdom

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2010
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60761-460-9
  • Online ISBN 978-1-60761-461-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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