Overview
- Covers a wide range of live-cell imaging techniques
- Detailed methods for SEM and TEM analysis of cytoskeleton components
- Techniques for imaging the plant cytoskeleton
- Cutting-edge protocols for quantitative fluorescence microscopy
- Presents proteomic tools for cytoskeleton research
- Comprehensive protocols for qualitative and quantitative analyses of cell and organelle motility
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 586)
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Table of contents (22 protocols)
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Analysis of Cell and Organelle Motility
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Genetic and Proteomics Approaches for Cytoskeleton Protein Function
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Front Matter
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Back Matter
Keywords
About this book
In the ten years since the publication of the first edition, great advances in fluorescent labeling, optics, and sample preparation have significantly improved the imaging capability of microscopy, allowing for a continual refinement of our understanding of the cytoskeleton as a dynamic synergy of components. In Cytoskeleton Methods and Protocols, Second Edition, internationally renowned experts present techniques which reflect many of the recent technological advances in experimental tools for cytoskeleton research with emphasis on animal, plant, protist, and fungal model systems. This cutting-edge volume contains methods for live-cell imaging, fluorescence microscopy, electron microscopy, analysis of cell and organelle motility, isolation of cytoskeleton components, and proteomics, amongst other topics. As a volume in the highly successful Methods in Molecular Biology™ series, chapters incorporate introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes that provide unpublished technical information on troubleshooting and avoiding known pitfalls.
Up-to-date and comprehensive, Cytoskeleton Methods and Protocols, Second Edition serves as an ideal guide to scientists who wish to continue this fruitful and important biological research.
Reviews
From the reviews of the second edition:
“In this second edition it is particularly stressed the cytoskeleton study at the different levels of life organization … . chapters incorporate a clear introduction, a useful lists of the necessary materials and reagents and the usual (so appreciated by beginners) step-by-step laboratory protocols to avoid the troubleshooting and pitfalls of the bench journey. Cell and developmental biologists are the primary targets as an audience … .” (Carlo Alberto Redi, European Journal of Histochemistry, Spring, 2010)
“The book contains an interesting mix of chapters some of which cover basic concepts and ideas that are easily transferrable to other systems and others that are more specialised. … overall the book contains many useful nuggets of information and will be very useful for anyone wanting to try out a new technique or approach covered by the various chapters.” (Michelle Peckham, Infocus Magazine, Issue 21, March, 2011)
“This second edition of Cytoskeleton Methods and Protocols … has the chapters written by different authors. Those authors which have highly experienced and are widely recognized as experts in their fields show in this book detailed protocols combining biochemistry, immunology, genetics, microscopy, and image analysis with integrative approaches for investigations of cytoskeleton components. Each protocol is provided with step-by-step instructions and informative notes that lead both novice and experienced investigators to a successful use. … serve as an ideal guide to different kind of investigators.” (Ricardo Ambrósio Fock, Brazilian Journal of Pharmaceutical Sciences, Vol. 47 (2), Summer, 2011)
Editors and Affiliations
Bibliographic Information
Book Title: Cytoskeleton Methods and Protocols
Editors: Ray H. Gavin
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-60761-376-3
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2010
Hardcover ISBN: 978-1-60761-375-6Published: 02 October 2009
Softcover ISBN: 978-1-61779-649-4Published: 12 July 2012
eBook ISBN: 978-1-60761-376-3Published: 18 September 2009
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: XII, 391
Number of Illustrations: 77 b/w illustrations
Additional Information: Originally published as volume 161 in the series: Methods in Molecular Biology
Topics: Biochemistry, general, Cell Biology, Protein Science, Membrane Biology, Biological Microscopy
Industry Sectors: Biotechnology, Consumer Packaged Goods, Pharma