© 2010

Cytoskeleton Methods and Protocols

  • Ray H. Gavin

Part of the Methods in Molecular Biology book series (MIMB, volume 586)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Live-Cell Imaging of the Cytoskeleton

    1. Front Matter
      Pages 1-1
    2. Sarah Woolner, Ann L. Miller, William M. Bement
      Pages 23-39
  3. Dynamics of Actin Filaments and Microtubules

    1. Front Matter
      Pages 70-70
  4. Fluorescence Microscopy Protocols for Investigating Cytoskeleton Components

    1. Front Matter
      Pages 116-116
    2. Alessandro Esposito, Simon Schlachter, Gabriele S. Kaminski Schierle, Alan D. Elder, Alberto Diaspro, Fred S. Wouters et al.
      Pages 117-142
    3. Robert F. Hennigan, Mary F. Chaiken, Lauren A. Foster, Wallace Ip
      Pages 143-156
    4. Julia Dyachok, Cheol-Min Yoo, Karuppaiah Palanichelvam, Elison B. Blancaflor
      Pages 157-169
    5. Theresa C. Swayne, Istvan R. Boldogh, Liza A. Pon
      Pages 171-184
  5. Electron Microscopy Protocols for Imaging the Cytoskeleton

    1. Front Matter
      Pages 186-186
    2. Andreas Holzinger, Eiko Kawamura, Geoffrey O. Wasteneys
      Pages 243-262
  6. Analysis of Cell and Organelle Motility

    1. Front Matter
      Pages 264-264

About this book


In the ten years since the publication of the first edition, great advances in fluorescent labeling, optics, and sample preparation have significantly improved the imaging capability of microscopy, allowing for a continual refinement of our understanding of the cytoskeleton as a dynamic synergy of components. In Cytoskeleton Methods and Protocols, Second Edition, internationally renowned experts present techniques which reflect many of the recent technological advances in experimental tools for cytoskeleton research with emphasis on animal, plant, protist, and fungal model systems. This cutting-edge volume contains methods for live-cell imaging, fluorescence microscopy, electron microscopy, analysis of cell and organelle motility, isolation of cytoskeleton components, and proteomics, amongst other topics. As a volume in the highly successful Methods in Molecular Biology™ series, chapters incorporate introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes that provide unpublished technical information on troubleshooting and avoiding known pitfalls.

Up-to-date and comprehensive, Cytoskeleton Methods and Protocols, Second Edition serves as an ideal guide to scientists who wish to continue this fruitful and important biological research.


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Editors and affiliations

  • Ray H. Gavin
    • 1
  1. 1.Brooklyn CollegeCity University of New YorkNew YorkU.S.A.

Bibliographic information

  • Book Title Cytoskeleton Methods and Protocols
  • Editors Ray H. Gavin
  • Series Title Methods in Molecular Biology
  • Series Abbreviated Title Methods Molecular Biology
  • DOI
  • Copyright Information Humana Press 2010
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-60761-375-6
  • Softcover ISBN 978-1-61779-649-4
  • eBook ISBN 978-1-60761-376-3
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 2
  • Number of Pages XII, 391
  • Number of Illustrations 77 b/w illustrations, 0 illustrations in colour
  • Additional Information Originally published as volume 161 in the series: Methods in Molecular Biology
  • Topics Biochemistry, general
    Cell Biology
    Protein Science
    Membrane Biology
    Biological Microscopy
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From the reviews of the second edition:

“In this second edition it is particularly stressed the cytoskeleton study at the different levels of life organization … . chapters incorporate a clear introduction, a useful lists of the necessary materials and reagents and the usual (so appreciated by beginners) step-by-step laboratory protocols to avoid the troubleshooting and pitfalls of the bench journey. Cell and developmental biologists are the primary targets as an audience … .” (Carlo Alberto Redi, European Journal of Histochemistry, Spring, 2010)

“The book contains an interesting mix of chapters some of which cover basic concepts and ideas that are easily transferrable to other systems and others that are more specialised. … overall the book contains many useful nuggets of information and will be very useful for anyone wanting to try out a new technique or approach covered by the various chapters.” (Michelle Peckham, Infocus Magazine, Issue 21, March, 2011)

“This second edition of Cytoskeleton Methods and Protocols … has the chapters written by different authors. Those authors which have highly experienced and are widely recognized as experts in their fields show in this book detailed protocols combining biochemistry, immunology, genetics, microscopy, and image analysis with integrative approaches for investigations of cytoskeleton components. Each protocol is provided with step-by-step instructions and informative notes that lead both novice and experienced investigators to a successful use. … serve as an ideal guide to different kind of investigators.” (Ricardo Ambrósio Fock, Brazilian Journal of Pharmaceutical Sciences, Vol. 47 (2), Summer, 2011)