DNA Topoisomerases

Methods and Protocols

  • Duncan J. Clarke

Part of the Methods in Molecular Biology™ book series (MIMB, volume 582)

Table of contents

  1. Front Matter
    Pages i-x
  2. Duncan J. Clarke, Andrew Lane
    Pages 1-9
  3. Omari J. Bandele, Neil Osheroff
    Pages 39-47
  4. Jan Lipfert, Daniel A. Koster, Igor D. Vilfan, Susanne Hage, Nynke H. Dekker
    Pages 71-89
  5. Rodrigo Bermejo, Yuki-Mori Katou, Katsuhiko Shirahige, Marco Foiani
    Pages 103-118
  6. Melissa Baldwin, Tariq Warsi, Jeff Bachant
    Pages 119-130
  7. Zbigniew Darzynkiewicz, Dorota H. Halicka, Toshiki Tanaka
    Pages 145-153
  8. Katherine Furniss, Amit C.J. Vas, Andrew Lane, Duncan J. Clarke
    Pages 167-187
  9. Melissa Baldwin, Jeff Bachant
    Pages 209-219
  10. John R. Daum, Yin Yuan Mo, Gary J. Gorbsky
    Pages 233-244
  11. Back Matter
    Pages 265-267

About this book


Due to their importance to cell biology, DNA topology-altering enzymes, collectively DNA topoisomerases and gyrases, have been studied thoroughly from a biochemical perspective, yet the expansion of research at the molecular and cellular levels continues to show a great deal of promise. In DNA Topoisomerases: Methods and Protocols, experts in the field present a compendium of protocols that extends into the new areas of research that have been recently uncovered in order to elucidate the methodology needed to study topoisomerases in the molecular and cellular context. The volume examines subjects such as the analysis of DNA topologies and the interaction of topoisomerases with DNA as tools to measure topoisomerase functions, the binding of topoisomerases to specific sites in the genomes of eukaryotes, including origins of DNA replication, as well as exploration of perturbed topoisomerase function. As a volume in the highly successful Methods in Molecular Biology™ series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, laboratory protocols, and notes on trouble-shooting and avoiding known pitfalls.

Cutting-edge and easy-to-use, DNA Topoisomerases: Methods and Protocols provides key content which reflects an expanded understanding of the functions of these essential enzymes in the hope of inspiring researchers to further important discoveries in this dynamic field.


Cellular functions Chromosom Chromosome structure DNA DNA replication DNA topologies Eukaryote genomes Post-translational modifications molecular biology

Editors and affiliations

  • Duncan J. Clarke
    • 1
  1. 1.Dept. Genetics, Cell Biology &University of Minnesota Medical SchoolMinneapolisU.S.A.

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2009
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60761-339-8
  • Online ISBN 978-1-60761-340-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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