Protocols for Neural Cell Culture

Fourth Edition

  • Laurie C. Doering

Part of the Springer Protocols Handbooks book series (SPH)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Sharon A. Louis, Brent A. Reynolds
    Pages 1-28
  3. Marcos R. Costa, Ravi Jagasia, Benedikt Berninger
    Pages 29-49
  4. Soojung Shin, Mohan Vemuri
    Pages 51-73
  5. Mirella Dottori, Alice Pébay, Martin F. Pera
    Pages 75-86
  6. Manon Blain, Veronique E. Miron, Caroline Lambert, Peter J. Darlington, Qiao-Ling Cui, Philippe Saikali et al.
    Pages 87-104
  7. Behnam A. Baghbaderani, Arindom Sen, Michael S. Kallos, Leo A. Behie
    Pages 105-123
  8. Navjot Kaur, David V. Thompson, David Judd, David R. Piper, Richard G. Del Mastro
    Pages 125-136
  9. Francine Nault, Paul De Koninck
    Pages 137-159
  10. Andrea M. Vincent, Eva L. Feldman
    Pages 161-173
  11. Francisco L.A.F. Gomes, Michel Cayouette
    Pages 175-191
  12. Jean de Vellis, Cristina A. Ghiani, Ina B. Wanner, Ruth Cole
    Pages 193-215
  13. Akiko Nishiyama, Ryusuke Suzuki, Hao Zuo, Xiaoqin Zhu
    Pages 217-230
  14. Makoto Sawada, Hiromi Suzuki
    Pages 231-239
  15. Sergey Fedoroff, Arleen Richardson
    Pages 241-251
  16. Haesun A. Kim, Patrice Maurel
    Pages 253-268
  17. Shelley Jacobs, Laurie C. Doering
    Pages 269-283
  18. Josef P. Kapfhammer
    Pages 285-298
  19. Jesse E. Hanson, Adrienne L. Orr, Silvia Fernandez-Illescas, Ricardo A. Valenzuela, Daniel V. Madison
    Pages 299-311
  20. Saulius Satkauskas, Arnaud Muller, Morgane Roth, Dominique Bagnard
    Pages 313-327
  21. Sean P. Cregan
    Pages 343-351
  22. Sabine Bavamian, Eliana Scemes, Paolo Meda
    Pages 353-373
  23. Arleen Richardson, Sergey Fedoroff
    Pages 375-390
  24. Back Matter
    Pages 391-400

About this book


Since the conception of this acclaimed series of volumes examining neural tissue culture, the expansion of neuroscience has continued to produce vital discoveries that utilize tissue culture methodologies.  The expert contributors to the fourth edition of Protocols for Neural Cell Culture refine existing protocols and present the emerging new techniques and culture media formulations linked with the many advances in neuroscience.  While highlighting updated experimental procedures for many of the classical neural tissue culture preps, the volume addresses topics such as regenerative medicine and the methods to grow and expand embryonic and adult neural stem cells. Essential techniques for the isolation, expansion, and cryopreservation of neural tissue from mouse, rat, and human sources are covered, as well as methods to assess aspects of cell function (calcium imaging) and cell death.  With its detailed, step-by-step laboratory protocols, this volume provides the kind of detailed description and implementation advice that is crucial for getting optimal results.

Authoritative and cutting-edge, Protocols for Neural Cell Culture, Fourth Edition continues the  previous editions’ tradition of exceptional quality and thorough coverage in tissue culture protocols for today’s neuroscience in order to inspire researchers in the field to further explore this imperative area of study.


Calcium imaging Cell death Immunocytochemistry Neuroscience Regenerative medicine Stem cells cryopreservation neurons

Editors and affiliations

  • Laurie C. Doering
    • 1
  1. 1.Dept. Pathology & Molecular MedicineMcMaster UniversityHamiltonCanada

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2010
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60761-291-9
  • Online ISBN 978-1-60761-292-6
  • Series Print ISSN 1949-2448
  • Series Online ISSN 1949-2456
  • Buy this book on publisher's site
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