Methods and Protocols

  • Jörg Reinders
  • Albert Sickmann

Part of the Methods in Molecular Biology™ book series (MIMB, volume 564)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Introduction

    1. Front Matter
      Pages 1-1
    2. Friedrich Lottspeich
      Pages 3-10
  3. Electrophorestic Separations

    1. Front Matter
      Pages 12-12
    2. Walter Weiss, Angelika Görg
      Pages 13-32
    3. Jacqueline Burré, Ilka Wittig, Hermann Schägger
      Pages 33-57
    4. Walter Weiss, Florian Weiland, Angelika Görg
      Pages 59-82
  4. Mass Spectrometry and Tandem Mass Spectrometry Applications

    1. Front Matter
      Pages 84-84
    2. Rainer Cramer
      Pages 85-103
    3. Thomas Fröhlich, Georg J. Arnold
      Pages 123-141
    4. Katharina Lohrig, Dirk Wolters
      Pages 143-153
    5. Oliver Simon, Stefanie Wortelkamp, Albert Sickmann
      Pages 155-171
    6. Reinhold Weber, Regina Preywisch, Nikolay Youhnovski, Marcus Groettrup, Michael Przybylski
      Pages 173-186
  5. Quantitative Proteomics

    1. Front Matter
      Pages 188-188
    2. Michael W. Linscheid, Robert Ahrends, Stefan Pieper, Andreas Kühn
      Pages 189-205
  6. Interpretation of Mass Spectrometry Data

    1. Front Matter
      Pages 244-244
    2. Lennart Martens, Rolf Apweiler
      Pages 245-259
    3. Bingwen Lu, Tao Xu, Sung Kyu Park, John R. Yates III
      Pages 261-288
  7. Analysis of Protein Modifications

    1. Front Matter
      Pages 290-290
    2. Rukhsana Sultana, Marzia Perluigi, D. Allan Butterfield
      Pages 291-301
  8. Subcellular Proteomics

  9. Analysis of Protein Interactions

    1. Front Matter
      Pages 358-358
    2. Christian Johannes Gloeckner, Karsten Boldt, Annette Schumacher, Marius Ueffing
      Pages 359-372
    3. Mohan Babu, Gareth Butl, Oxana Pogoutse, Joyce Li, Jack F. Greenblatt, Andrew Emili
      Pages 373-400
    4. Beate Krüger, Thomas Dandekar
      Pages 401-431
  10. Back Matter
    Pages 433-441

About this book


Since the development of the concept of "proteomics," great progress has granted deeper insight into the cellular network, but the enormous range of protein abundance, dynamics, and interactions leaves many further challenges in the field. In Proteomics: Methods and Protocols, experts in proteomic research have contributed detailed techniques, which present an overview of the contemporary challenges and possibilities in the various areas of proteomics, as well as cutting-edge protocols that provide concrete examples for successful proteome analysis. Written in the clear and concise Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easy-to-use, Proteomics: Methods and Protocols will serve as a valuable reference book for laboratories around the world and raise interest in the complex and vital field of proteomics research.


Cellular networks Electrophorestic separations Escherichia coli Organelle Protein interactions Protein modifications Proteomics Quantitative and subcellular proteomics Tandem mass spectrometry membrane proteins molecular biology protein complexes

Editors and affiliations

  • Jörg Reinders
  • Albert Sickmann

There are no affiliations available

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2009
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60761-156-1
  • Online ISBN 978-1-60761-157-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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