Methods and Protocols

  • Andrew D. McAinsh

Part of the Methods in Molecular Biology book series (MIMB, volume 545)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Eli Berdougo, Marie-Emilie Terret, Prasad V. Jallepalli
    Pages 21-37
  3. Mónica Bettencourt-Dias, Gohta Goshima
    Pages 39-62
  4. Sarah E. McClelland, Andrew D. McAinsh
    Pages 81-98
  5. Pier Paolo D’Avino, Vincent Archambault, Marcin R. Przewloka, Wei Zhang, Ernest D. Laue, David M. Glover
    Pages 99-112
  6. Eileen O’Toole, Thomas Müller-Reichert
    Pages 135-144
  7. António J. Pereira, Irina Matos, Mariana Lince-Faria, Helder Maiato
    Pages 145-164
  8. Suzanna L. Prosser, Andrew M. Fry
    Pages 165-183
  9. Christiane Klebig, Alberto Toso, Satyarebala Borusu, Patrick Meraldi
    Pages 205-220
  10. Michael J. Emanuele, P. Todd Stukenberg
    Pages 221-232
  11. Catherine Lindon, Barbara Di Fiore
    Pages 259-270
  12. Hiroyuki Yamano, Michelle Trickey, Margaret Grimaldi, Yuu Kimata
    Pages 287-300
  13. Adam Williamson, Lingyan Jin, Michael Rape
    Pages 301-312
  14. Back Matter
    Pages 126-345

About this book


With the advent of modern methodology, the phenomenal complexity of the protein components and regulatory steps involved in mitosis has become approachable. In Mitosis: Methods and Protocols, experts in the field provide an up-to-date collection of methods and approaches that are used to investigate the mechanism of mitosis at the molecular level. Ideal for both experienced and novice scientists, the volume includes step-by-step methods covering inactivation of genes of interest, depletion of proteins of interest, biochemical and microscope-based techniques, and procedures to monitor and measure key mitotic processes. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to the respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Comprehensive and authoritative, Mitosis: Methods and Protocols combines imaging, biochemical, and genetic methodologies in order to best guide researchers in their attempts to better understand the crucial processes of mitotic regulation.


Activation Drosophila Promoter Xenopus laevis cell lines genes molecular biology proteins regulation

Editors and affiliations

  • Andrew D. McAinsh
    • 1
  1. 1.Chromosome Segregation Lab.Marie Curie Research InstituteOxted, SurreyUnited Kingdom

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2009
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60327-992-5
  • Online ISBN 978-1-60327-993-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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