Methods and Protocols

  • Graham J. Lieschke
  • Andrew C. Oates
  • Koichi Kawakami

Part of the Methods in Molecular Biology book series (MIMB, volume 546)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Mutagenesis and Mutants

    1. Front Matter
      Pages 1-1
    2. Ewart de Bruijn, Edwin Cuppen, Harma Feitsma
      Pages 3-12
    3. Carrie Carmichael, Monte Westerfield, Zoltán M. Varga
      Pages 45-65
  3. Transgenesis

    1. Front Matter
      Pages 68-68
    2. Hiroshi Kikuta, Koichi Kawakami
      Pages 69-84
    3. Zhongan Yang, Hong Jiang, Shuo Lin
      Pages 103-116
    4. Daniele Soroldoni, Benjamin M. Hogan, Andrew C. Oates
      Pages 117-130
  4. Tissue-Specific Manipulations

    1. Front Matter
      Pages 132-132
    2. Harshan Pisharath, Michael J. Parsons
      Pages 133-143
    3. Marcel Tawk, Isaac H. Bianco, Jonathan D. W. Clarke
      Pages 145-151
    4. Alexander Picker, Daniela Roellig, Olivier Pourquié, Andrew C. Oates, Michael Brand
      Pages 153-172
  5. Analyzing Gene Expression

    1. Front Matter
      Pages 174-174
    2. Siew Hong Lam, R. Krishna Murthy Karuturi, Zhiyuan Gong
      Pages 197-226
    3. Luke Pase, Graham J. Lieschke
      Pages 227-240
  6. Imaging

    1. Front Matter
      Pages 242-242
    2. Leah Herrgen, Christian Schröter, Lola Bajard, Andrew C. Oates
      Pages 243-254
    3. Chris Hall, Maria Vega Flores, Kathy Crosier, Phil Crosier
      Pages 255-271
    4. Lara Carvalho, Carl-Philipp Heisenberg
      Pages 273-287
    5. Andrew J. Trotter, Adam C. Parslow, Joan K. Heath
      Pages 289-315
  7. Back Matter
    Pages 333-335

About this book


In the last 20 years, research activity using the zebrafish Danio rerio has increased dramatically, due in part to the ease of breeding and raising them, their genetic tractability, embryonic accessibility, and their imaging potential. In Zebrafish: Methods and Protocols, expert researchers provide a collection of experimental techniques for the genetic modification, manipulation, and in vivo live imaging of zebrafish. This volume collects sophisticated and practical techniques, including several not yet widely known, from laboratories around the world. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and accessible, Zebrafish: Methods and Protocols seeks to aid and inspire scientists utilizing this versatile animal model in their research.


Chromosome Microarray cryopreservation gene expression genes hybridization microscopy molecular biology mutant

Editors and affiliations

  • Graham J. Lieschke
    • 1
  • Andrew C. Oates
    • 2
  • Koichi Kawakami
    • 3
  1. 1.Walter & Eliza Hall Institute of, Medical ResearchRoyal Melbourne HospitalParkvilleAustralia
  2. 2.und GenetikMPI für Molekulare ZellbiologieDresdenGermany
  3. 3.Div. Molecular & Developmental, BiologyNational Institute of GeneticsShizuokaJapan

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2009
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60327-976-5
  • Online ISBN 978-1-60327-977-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site