Methods and Protocols

  • Marjo de Graauw

Part of the Methods in Molecular Biology™ book series (MIMB, volume 527)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Protein Phosphorylation and 2D Gel Electrophoresis

  3. Enrichment of Phosphoproteins and Peptides

  4. Phosphoprotein Analysis by Mass Spectrometry

    1. Angela Amoresano, Claudia Cirulli, Gianluca Monti, Eric Quemeneur, Gennaro Marino
      Pages 173-190
    2. Ralf Krüger, Nico Zinn, Wolf D. Lehmann
      Pages 201-218
    3. Kris Gevaert, Joël Vandekerckhove
      Pages 219-227
    4. Alexander Leitner, Wolfgang Lindner
      Pages 229-243
  5. Arrays to Study Protein-Phosphorylation

  6. Bioinformatics

  7. Back Matter
    Pages 321-328

About this book


Protein phosphorylation controls many basic cellular processes, such as cell growth, differentiation, migration, metabolism, and cell death, and its study can provide key insights into the signal transduction pathways that are activated in cells in response to different stimuli, such as growth factor stimulation or exposure to toxicants. In Phospho-Proteomics: Methods and Protocols, expert researchers contribute both well-established protocols and some of the newest strategies for the identification and evaluation of protein phosphorylation on Tyr, Ser, and Thr residues, including topics such as 2-dimensional gel electrophoresis and protein phosphorylation, enrichment of phospho-proteins and peptides, quantitative analysis of phosphorylation by labeling and MS analysis, and antibody and kinase arrays. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Phospho-Proteomics: Methods and Protocols is an ideal reference for both new and experienced scientists who wish to gain insight into the current developments in the field and to find inspiration to pursue its future.


2D gel electrophoresis Enrichment Mass Spectrometry Phosphorylated proteins Phosphorylation Proteomics Signal transduction pathways bioinformatics cellular processes migration proteins

Editors and affiliations

  • Marjo de Graauw
    • 1
  1. 1.Division of Toxicology, Leiden/Amsterdam Center for Drug Research (LACDR)Leiden UniversityLeidenNetherlands

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2009
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60327-833-1
  • Online ISBN 978-1-60327-834-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
Industry Sectors
Consumer Packaged Goods