The Nuclear Receptor Superfamily

Methods and Protocols

  • Iain J. McEwan

Part of the Methods in Molecular Biology™ book series (MIMB, volume 505)

Table of contents

  1. Front Matter
    Pages i-x
  2. Introduction

    1. Iain J. McEwan
      Pages 3-18
  3. Ligand Binding and Nuclear Receptor Turnover

  4. Nuclear Localization and DNA Binding

    1. Martin E. van Royen, Christoffel Dinant, Pascal Farla, Jan Trapman, Adriaan B. Houtsmuller
      Pages 69-96
    2. Jason T. Read, Helen Cheng, Stephen C. Hendy, Colleen C. Nelson, Paul S. Rennie
      Pages 97-122
    3. Charles E. Massie, Ian G. Mills
      Pages 123-137
  5. Nuclear Receptor—Co-Regulatory Protein Interactions

  6. Pathophysiological Analysis of Nuclear Receptor Function

    1. Inés Pineda Torra, Julia A. Staverosky, Susan Ha, Susan K. Logan, Michael J. Garabedian
      Pages 221-235
    2. Outi R. Saramäki, Kati K. Waltering, Tapio Visakorpi
      Pages 263-277
  7. Back Matter
    Pages 279-281

About this book


Acting principally to control patterns of gene expression, nuclear receptors play vital roles during embryonic development and in the regulation of metabolic and reproductive functions in adult life, which proves this superfamily of ligand-activated transcription factors to be a crucial part of biological life.  In The Nuclear Receptor Superfamily: Methods and Protocols, expert researchers describe a range of molecular, structural and cell biological techniques currently used to investigate the structure-function of nuclear receptors, together with experimental approaches that may lead to new therapeutic strategies for treating nuclear receptor-associated diseases.  Written in the highly successful Methods in Molecular Biology™ series format, the chapters in this volume contain brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, as well as notes from the experts to highlight tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and easy to use, The Nuclear Receptor Superfamily: Methods and Protocols provides beneficial and time-saving guidance for all those undertaking research in this ever-growing field of study.


Chaperone Cloning DNA DNA binding Ligand binding Natural ligands Protein interactions Regulation Steroid hormones gene expression leukocytes molecular biology transcription

Editors and affiliations

  • Iain J. McEwan
    • 1
  1. 1.School of Medical SciencesUniversity of AberdeenAberdeenUK

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2009
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60327-574-3
  • Online ISBN 978-1-60327-575-0
  • Series Print ISSN 1064-3745
  • Buy this book on publisher's site
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