Overview
- Provides an excellent source for understanding the strengths and weaknesses of the various vertebrate model organisms
- Comprehensive guide to the cutting-edge methods amongst the dramatically growing field of vertebrate molecular embryology
- Experts in the field thoroughly describe protocols and techniques accompanied by illustrations
- New approaches for efficient production of transgenic zebrafish and microarrays
Part of the book series: Methods in Molecular Biology (MIMB, volume 461)
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Table of contents (54 protocols)
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The Chicken Embryo
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Nonvertebrate Chordates
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Molecular Techniques
Keywords
About this book
In Molecular Embryology: Methods and Protocols, Second Edition, expert investigators provide a comprehensive guide to the cutting-edge methods used across the dramatically growing field of vertebrate molecular embryology. Time-tested techniques take advantage of the most commonly used vertebrate experimental models: murine embryos for their genetics, chick embryos for in vivo manipulation, zebrafish for mutagenesis, amphibian embryos, and nonvertebrate chordates. The second edition collects classic protocols which have become standard techniques in the laboratory and presents them in a complementary fashion with novel and emerging approaches, allowing researcher to become more familiar with commonly studied embryos used in biomedical research. Insightful to the experienced professional, Molecular Embryology: Methods and Protocols, Second Edition, presents cutting-edge findings of perhaps the greatest period in growth and productivity in the field of developmental biology.
Reviews
Reviews of the first edition:
"This book has much to offer biologists interested in the molecular biology of developmental systems in a wide range of vertebrates." - Doody's Health Sciences Book Review Journal
"Universal techniques are well-written, complete, and easy to follow including in situ hybridization, differential display PCR, as well as standard protocols for library construction, Northern and Southern analyses. This would be an excellent addition to any developmental biology laboratory, university department, and especially good for graduate students and post-docs." -Teratology
"...this book is a tome, a necessity imposed on it by its breadth, both in terms of model organisms (mouse, chicken, amphibians, zebra fish and prochordates) and methods (surgery, retro-virology, in situ hybridization, molecular biology, immunohistochemistry, microscopy and photography.) The protocols are compact and clearly written, with ever-so-helpful hints (called notes) grouped at the end of each chapter. ...we encourage both teachers and researchers actively involved in experimental embryology to buy this book, put it on the shelf and let your students know it is there." - TINS
"Molecular Embryology provides an excellent source for understanding the strengths and weaknesses of the various vertebrate model organisms, and provides a ready source for highly useful protocols....The protocols are thoroughly described and are accompanied by helpful illustrations. Developmental biologists will be pleased to add this book to their collection, and will likely reference it often for its many useful protocols." - The Quarterly Review of Biology
"This book is of interest to embryologists, biologists and probably to some neonatologists and geneticists. It describes in detail the experimental use of animal embryos...A well illustrated and referenced text for specialists." - Journal of Endocrine Genetics
Editors and Affiliations
Bibliographic Information
Book Title: Molecular Embryology
Book Subtitle: Methods and Protocols
Editors: Paul T. Sharpe, Ivor Mason
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-60327-483-8
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2009
Hardcover ISBN: 978-1-58829-431-9Published: 20 October 2008
Softcover ISBN: 978-1-61737-565-1Published: 19 November 2010
eBook ISBN: 978-1-60327-483-8Published: 23 November 2008
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: XVII, 798
Topics: Biochemistry, general, Cell Biology, Developmental Biology
Industry Sectors: Biotechnology, Consumer Packaged Goods, Pharma