DNA Damage Detection In Situ, Ex Vivo, and In Vivo

Methods and Protocols

  • Vladimir V. Didenko

Part of the Methods in Molecular Biology book series (MIMB, volume 682)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Detection in Tissue Sections

  3. Detection in Cell Cultures

    1. Front Matter
      Pages 89-89
    2. Zbigniew Darzynkiewicz, Piotr Pozarowski, Brian W. Lee, Gary L. Johnson
      Pages 103-114
    3. José Luis Fernández, Dioleyda Cajigal, Jaime Gosálvez
      Pages 133-147
    4. Jennifer Guerrero-Santoro, Arthur S. Levine, Vesna Rapić-Otrin
      Pages 149-161
  4. Detection in Live Tissues, Blood, Urine, Sperm

    1. Front Matter
      Pages 163-163
    2. Roxana M. Vlad, Michael C. Kolios, Gregory J. Czarnota
      Pages 165-187
    3. Jagadeesan Nair, Urmila J. Nair, Xin Sun, Ying Wang, Khelifa Arab, Helmut Bartsch
      Pages 189-205
    4. Jodi Hagen, Jeffrey P. Houchins, Alexander E. Kalyuzhny
      Pages 207-215
    5. Philip Thomas, Michael Fenech
      Pages 217-234
    6. Philip Thomas, Michael Fenech
      Pages 235-248
    7. Christophe E. Redon, Asako J. Nakamura, Olivier Sordet, Jennifer S. Dickey, Ksenia Gouliaeva, Brian Tabb et al.
      Pages 249-270
    8. Regina M. Santella, Yu-Jing Zhang
      Pages 271-278
    9. Vilas Mistry, Friederike Teichert, Jatinderpal K. Sandhu, Rajinder Singh, Mark D. Evans, Peter B. Farmer et al.
      Pages 279-289
    10. José Luis Fernández, Dioleyda Cajigal, Carmen López-Fernández, Jaime Gosálvez
      Pages 291-301
    11. Philip Thomas, Michael Fenech
      Pages E1-E1
  5. Back Matter
    Pages 303-313

About this book


Recent advances in organic chemistry, fluorescent microscopy, and materials science have created an entirely new range of techniques and probes for imaging DNA damage in molecular and cellular biology. In DNA Damage Detection In Situ, Ex Vivo, and In Vivo: Methods and Protocols, expert researchers explore the latest advances in the area, covering both recent and established techniques to detect and quantify DNA damage at scales ranging from subcellular to the level of a whole live organism. Chapters present all major assays used in molecular and cellular biology for the labeling of DNA damage in situ, ex vivo, and in vivo. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and current, DNA Damage Detection In Situ, Ex Vivo, and In Vivo: Methods and Protocols is an essential handbook for novice and experienced researchers in a variety of fields, including molecular and cellular biology, experimental and clinical pathology, toxicology, radiobiology, oncology, embryology, experimental pharmacology, drug design, and environmental science.

Editors and affiliations

  • Vladimir V. Didenko
    • 1
  1. 1.Baylor College of Medicine, Depts. Neurosurgery and Molecular & CellUniversity of HoustonHoustonUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60327-408-1
  • Online ISBN 978-1-60327-409-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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