Rat Genomics

Methods and Protocols

  • Ignacio Anegon

Part of the Methods in Molecular Biology book series (MIMB, volume 597)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Timothy J. Aitman, Enrico Petretto, Jacques Behmoaras
    Pages 13-32
  3. Elizabeth A. Worthey, Alexander J. Stoddard, Howard J. Jacob
    Pages 33-53
  4. Séverine Ménoret, Séverine Remy, Claire Usal, Laurent Tesson, Ignacio Anegon
    Pages 81-92
  5. Ri-ichi Takahashi, Masatsugu Ueda
    Pages 93-108
  6. Séverine Remy, Tuan Huy Nguyen, Séverine Ménoret, Laurent Tesson, Claire Usal, Ignacio Anegon
    Pages 109-125
  7. Jean Cozzi, Eryao Wang, Christelle Jacquet, Alexandre Fraichard, Yacine Cherifi, Qi Zhou
    Pages 137-150
  8. Ruben van Boxtel, Michael N. Gould, Edwin Cuppen, Bart M. G. Smits
    Pages 151-167
  9. Masaki Kawamata, Takahiro Ochiya
    Pages 169-177
  10. Aron M. Geurts, Gregory J. Cost, Séverine Rémy, Xiaoxia Cui, Laurent Tesson, Claire Usal et al.
    Pages 211-225
  11. Nan Mei, James C. Fuscoe, Edward K. Lobenhofer, Lei Guo
    Pages 227-241
  12. Dominique Lagrange, Gilbert J Fournié
    Pages 243-266
  13. Laurent Tesson, Séverine Rémy, Séverine Ménoret, Claire Usal, Ignacio Anegon
    Pages 277-285

About this book

Introduction

Grown exponentially by the genomic revolution, the use of the rat as a model of choice for physiological studies continues in popularity and at a much greater depth of understanding. In Rat Genomics: Methods and Protocols, world-wide experts provide both practical information for researchers involved in genomic research in the rat along with a more contextual discussion about the usefulness of the rat in physiological or translational research in different organs and systems. The volume extensively covers topics including genome sequencing, quantitative trait loci mapping, and the identification of single nucleotide polymorphisms as well as the development of transgenic technologies such as nuclear cloning, lentiviral-mediated transgenesis, gene knock-down using RNA interference, gene knock-out by mutagenesis, and zinc finger nucleases plus exciting advances in the obtention of rat embryonic cell lines. As a volume in the highly successful Methods in Molecular Biology™ series, this work provides the kind of detailed description and implementation advice that is crucial for getting optimal results.

Comprehensive and up-to-date, Rat Genomics: Methods and Protocols thoroughly covers the current techniques used in labs around the world and overviews the applications of the data obtained, making it certain to be useful to the scientific community as a key source of references and methods.

Keywords

BAC DNA Microarray PCR Promoter Quantitative PCR Single Nucleotide Polymorphism Translation YAC cell lines cells cryopreservation gene expression molecular biology transgenic animals

Editors and affiliations

  • Ignacio Anegon
    • 1
  1. 1.ITERT-INSERM U643CHU de NantesNantesFrance

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-60327-389-3
  • Copyright Information Humana Press 2010
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60327-388-6
  • Online ISBN 978-1-60327-389-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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