Lipoproteins and Cardiovascular Disease

Methods and Protocols

  • Lita A. Freeman

Part of the Methods in Molecular Biology book series (MIMB, volume 1027)

Table of contents

  1. Front Matter
    Pages i-x
  2. RNA and Gene Expression

  3. Sequencing

    1. Front Matter
      Pages 137-137
    2. Victoria Youngblood, James G. Taylor VI
      Pages 139-155
    3. Lena Diaw, Victoria Youngblood, James G. Taylor VI
      Pages 157-179
  4. Transgenic, Knockout, and Knockdown Methodologies

    1. Front Matter
      Pages 181-181
    2. Chengyu Liu, Yubin Du, Wen Xie, Changyun Gui
      Pages 203-215
    3. Chengyu Liu, Wen Xie, Changyun Gui, Yubin Du
      Pages 217-232
    4. William R. Lagor, Julie C. Johnston, Martin Lock, Luk H. Vandenberghe, Daniel J. Rader
      Pages 273-307
  5. Special Topics

    1. Front Matter
      Pages 325-325
    2. Grant D. Barish, Rajenda K. Tangirala
      Pages 327-342
    3. Lita A. Freeman
      Pages 369-385
  6. Back Matter
    Pages 387-391

About this book


The lipid-rich and otherwise challenging nature of many key tissues complicates many aspects of current research, and applications of the unique nature of lipoproteins and their biological effects has engendered unique and vital methodologies.  In Lipoproteins and Cardiovascular Disease: Methods and Protocols, experts in the field present a compendium of advanced and classical molecular biology methods targeted towards lipoprotein, atherosclerosis, and vascular biology research, bringing together in a single volume an updated set of protocols and strategies for methods now driving the most recent advances, along with classical methods that are still widely used.  Among the many topics covered in this cutting-edge work, the book delves into crucial techniques such as quantitative real-time PCR, microarrays, RT-PCR laser capture microdissection, and tissue-specific gene overexpression, knockout, and knockdown methodologies, including AAV as a liver-directed gene delivery vehicle.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and valuable notes which highlight tips on troubleshooting and avoiding known pitfalls.


Comprehensive and easy to use, Lipoproteins and Cardiovascular Disease: Methods and Protocols serves both novices and experts alike as a complete guide for any researcher with an interest in lipoproteins and their significant biological effects.


Atherosclerosis Gene knockout Lipoprotein RNA and gene expression Sequencing Transgenic models Vascular biology research

Editors and affiliations

  • Lita A. Freeman
    • 1
  1. 1.Pulmonary & Vascular Medicine BranchNational Institutes of Health (NIH) National Heart, Lung & Blood InstituteBethesdaUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60327-368-8
  • Online ISBN 978-1-60327-369-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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