Clostridium difficile

Methods and Protocols

  • Peter Mullany
  • Adam P. Roberts

Part of the Methods in Molecular Biology™ book series (MIMB, volume 646)

Table of contents

  1. Front Matter
    Pages i-x
  2. Introduction to Clostridium difficile and the Disease It Causes

    1. Front Matter
      Pages 1-1
    2. Adam P. Roberts, Peter Mullany
      Pages 3-7
  3. Isolation and Culture Techniques

    1. Front Matter
      Pages 37-37
  4. Methods for Typing and Epidemiological Studies

    1. Front Matter
      Pages 53-53
    2. Maja Rupnik
      Pages 67-76
    3. Ludovic Lemée, Jean-Louis Pons
      Pages 77-90
  5. Biochemistry of the Organism

    1. Front Matter
      Pages 91-91
    2. Robert Fagan, Neil Fairweather
      Pages 117-134
    3. Nazila V. Jafari, Elaine Allan, Mona Bajaj-Elliott
      Pages 135-146
  6. Genomics

    1. Front Matter
      Pages 147-147
    2. Richard Stabler, Lisa Dawson, Brendan Wren
      Pages 149-162
  7. Development of Systems for Genetic Analysis of the Organism

    1. Front Matter
      Pages 163-163
    2. John T. Heap, Stephen T. Cartman, Sarah A. Kuehne, Clare Cooksley, Nigel P. Minton
      Pages 165-182
    3. Glen P. Carter, Dena Lyras, Rachael Poon, Pauline M. Howarth, Julian I. Rood
      Pages 183-201
    4. Haitham A. Hussain, Adam P. Roberts, Rachael Whalan, Peter Mullany
      Pages 203-211
  8. Animal Models of Disease

    1. Front Matter
      Pages 213-213
    2. Gillian Douce, David Goulding
      Pages 215-227
    3. Anne Collignon
      Pages 229-237
  9. Back Matter
    Pages 239-241

About this book


Clostridium difficile, a major nosocomial pathogen shown to be a primary cause of antibiotic-associated disease, has emerged as a highly transmissible and frequently antibiotic-resistant organism, causing a considerable burden on health care systems worldwide. In Clostridium difficile: Methods and Protocols, expert researchers bring together the most recently developed methods for studying the organism, including techniques involving isolation, molecular typing, genomics, genetic manipulation, and the use of animal models.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes highlighting tips on troubleshooting and avoiding known pitfalls.


Authoritative and cutting-edge, Clostridium difficile: Methods and Protocols serves as an ideal guide for scientists now in a position to gain an in-depth understanding of how this organism is transmitted and how it causes disease.


DNA Microarray PCR Toxin antibiotic-associated disease antibiotics genes molecular biology

Editors and affiliations

  • Peter Mullany
    • 1
  • Adam P. Roberts
    • 1
  1. 1.Eastman Dental Institute Division of Microbial DiseasesUniversity College, LondonLondonUK

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2010
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60327-364-0
  • Online ISBN 978-1-60327-365-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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