Histology Protocols

  • Tim D. Hewitson
  • Ian A. Darby

Part of the Methods in Molecular Biology book series (MIMB, volume 611)

Table of contents

  1. Front Matter
    Pages i-viii
  2. Tissue Processing

  3. Staining Techniques

    1. Front Matter
      Pages 41-41
    2. Su Ee Wong, Catherine E. Winbanks, Chrishan S. Samuel, Tim D. Hewitson
      Pages 103-114
    3. Andréa Monte-Alto-Costa, Luís Cristóvão Porto
      Pages 131-140
    4. Alexandra Petiet, G. Allan Johnson
      Pages 141-149
    5. Richard J. Young, Andreas Möller
      Pages 151-159
    6. Tim D. Hewitson, Ian A. Darby
      Pages 161-170
  4. Imaging Techniques

    1. Front Matter
      Pages 171-171
    2. Martin Stebbing, Simon Potocnik, Pinglu Ye, Emilio Badoer
      Pages 173-182
    3. Verena C. Wimmer, Andreas Möller
      Pages 183-191
    4. Carlos Alberto Mandarim-de-Lacerda, Caroline Fernandes-Santos, Marcia Barbosa Aguila
      Pages 211-225
  5. Back Matter
    Pages 1-3

About this book


With much of what we know about the pathogenesis of disease coming from the systematic and careful study of histological material, histology has proven to be an incredibly valuable area of study. In Histology Protocols, expert researchers in the field contribute detailed methods involving the preparation of tissue, providing an overview of fixation, embedding, and processing, along with the required techniques for the retrieval of RNA from histological sections, followed by sections on routine and specialist histological staining techniques, including immuno-, lectin, and hybridization histochemistry, histological staining, as well as specific methods for the in situ identification of hypoxia and apoptosis, and details on the advances in imaging and image analysis. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Histology Protocols will provide molecular biologists with the basic histochemical techniques and histologists with the molecular techniques necessary to realize the potential of their resource.


Histochemistry Histological material Imaging techniques Laboratory Pathogenesis Staining techniques Tissue processing electron microscopy gene expression genes hybridization molecular biology

Editors and affiliations

  • Tim D. Hewitson
    • 1
  • Ian A. Darby
    • 2
  1. 1.Dept. NephrologyRoyal Hospital MelbourneMelbourneAustralia
  2. 2.School of Medical Sciences, Cancer & Tissue Repair Research GroupRMIT UniversityMelbourneAustralia

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2010
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60327-344-2
  • Online ISBN 978-1-60327-345-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
Industry Sectors
Health & Hospitals