Methods and Protocols

  • Hui Sun
  • Gabriel H. Travis

Part of the Methods in Molecular Biology book series (MIMB, volume 652)

Table of contents

  1. Front Matter
    Pages i-x
  2. Maureen A. Kane, Joseph L. Napoli
    Pages 1-54
  3. Vladimir J. Kefalov, M. Carter Cornwall, Gordon L. Fain
    Pages 95-114
  4. James E. Evans, Peter McCaffery
    Pages 149-162
  5. Leslie J. Donato, Noa Noy
    Pages 177-187
  6. Marcin Golczak, Grzegorz Bereta, Akiko Maeda, Krzysztof Palczewski
    Pages 229-245
  7. Yoshikazu Imanishi, Krzysztof Palczewski
    Pages 247-261
  8. A. Catharine Ross
    Pages 295-313
  9. Gabriel H. Travis, Joanna Kaylor, Quan Yuan
    Pages 329-339
  10. Back Matter
    Pages 363-365

About this book


New and exciting biological functions are still being discovered for vitamin A derivatives, including the vast number of physiological activities of retinoids. In Retinoids: Methods and Protocols, expert researchers in the field present the most recent technical tools with diverse techniques for both in vitro and in vivo studies. Combining biochemical, biophysical, and cell biological techniques, the book addresses topics such as the detection and quantitation of retinoids using HPLC, mass spectrometry, and fluorescence, fluorescence anisotropy of retinol binding protein, cell culture models for studying retinoid transport and the role of retinol in embryonic stem cell culture, as well as many other detailed procedures. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes highlighting tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Retinoids: Methods and Protocols seeks to aid beginning and experienced researchers from widely varied fields in the search to uncover even more vital aspects of vitamin A’s impact on the human body.


Transport Vitamin Vivo biological functions cells enzymes hybridization molecular biology tissue

Editors and affiliations

  • Hui Sun
    • 1
  • Gabriel H. Travis
    • 2
  1. 1.David Geffen School of Medicine, Dept. PhysiologyUniversity of California, Los AngelesLos AngelesUSA
  2. 2., Jules Stein Eye InstituteUCLA School of MedicineLos AngelesUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2010
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60327-324-4
  • Online ISBN 978-1-60327-325-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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