Methods and Protocols

  • Preston B. Rich
  • Christelle Douillet

Part of the Methods in Molecular Biology™ book series (MIMB, volume 574)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Elisa Michelini, Luca Cevenini, Laura Mezzanotte, Aldo Roda
    Pages 1-13
  3. John Virostko, E. Duco Jansen
    Pages 15-23
  4. Lucia Seminario-Vidal, Eduardo R. Lazarowski, Seiko F. Okada
    Pages 25-36
  5. Angelina Contero, Edmond Richer, Ana Gondim, Ralph P. Mason
    Pages 37-45
  6. Daisuke Asanuma, Hisataka Kobayashi, Tetsuo Nagano, Yasuteru Urano
    Pages 47-62
  7. Byron Ballou, Lauren A. Ernst, Susan Andreko, James A. J. Fitzpatrick, B. Christoffer Lagerholm, Alan S. Waggoner et al.
    Pages 63-74
  8. Xiaojuan Chen, Dixon B. Kaufman
    Pages 75-85
  9. Akira Kanno, Yoshio Umezawa, Takeaki Ozawa
    Pages 105-114
  10. Javier S. Burgos
    Pages 115-124
  11. Kathryn E. Luker, Gary D. Luker
    Pages 125-135
  12. Siouxsie Wiles, Brian D. Robertson, Gad Frankel, Angela Kerton
    Pages 137-153
  13. Akira Kanno, Takeaki Ozawa, Yoshio Umezawa
    Pages 185-192
  14. Carlos Villalobos, María Teresa Alonso, Javier García-Sancho
    Pages 203-214
  15. Mélanie Robitaille, Isabelle Héroux, Alessandra Baragli, Terence E. Hébert
    Pages 215-234
  16. Lynn Mckeown, Vicky C. Jones, Owen T. Jones
    Pages 235-248
  17. Georges von Degenfeld, Tom S. Wehrman, Helen M. Blau
    Pages 249-259
  18. Back Matter
    Pages 261-267

About this book


Through the study and application of bioluminescence, scientists have painstakingly harnessed a powerful tool that enables us to seek a deeper understanding of the complex mechanisms underpinning so many vital biologic systems. In this fully revised and updated second edition of Bioluminescence: Methods and Protocols, expert researchers contribute a readable and utilitarian compilation of the newest and most innovative techniques that have emerged in this rapidly expanding and progressively diverse field including methods to assess cell trafficking, protein-protein interactions, intracellular signaling, and apoptosis. Also opening up the possibility to visualize and quantify biological mechanisms in real-time and in in vivo settings, the volume also describes the in vivo study of bacterial or viral infections, transplanted cells, stem cells proliferation, vascular flow, and tumors. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials, equipment, and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Bioluminescence: Methods and Protocols, Second Edition provides protocols that are detailed enough to be followed and adapted by scientific teams who have no previous expertise in bioluminescence in order to foster the potentially numerous breakthroughs and new applications from basic to applied science and medicine that must continue to be developed.


ATP Calcium Colon Expression Pathogen cells enzymes fluorescence gene expression genes imaging molecular biology

Editors and affiliations

  • Preston B. Rich
    • 1
  • Christelle Douillet
    • 2
  1. 1.Dept. SurgeryUniversity of North CarolinaChapel HillU.S.A.
  2. 2.Dept. SurgeryUniversity of North CarolinaChapel HillU.S.A.

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2009
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60327-320-6
  • Online ISBN 978-1-60327-321-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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