Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants

  • S. Mohan Jain
  • Praveen K. Saxena

Part of the Methods in Molecular Biology book series (MIMB, volume 547)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Micropropagation and Chemical Analysis

    1. Front Matter
      Pages 1-1
    2. Hely Häggman, Anna Maria Pirttilä, Karoliina Niemi, Tytti Sarjala, Riitta Julkunen-Tiitto
      Pages 35-52
    3. Chun-Zhao Liu, Praveen K. Saxena
      Pages 53-59
    4. Shashi B. Babbar, Neetika Walia, Amandeep Kaur
      Pages 61-70
    5. Laura Jaakola, Kaisu Riihinen, Hely Häggman, Anja Hohtola
      Pages 71-80
    6. S. Pillai Geetha, A. V. Raghu, Gerald Martin, Satheesh George, Indira Balachandran
      Pages 81-92
    7. Aneta Sabovljevic, Marko Sabovljevic, Nebojsa Jockovic
      Pages 117-128
    8. Sushma Koul, K. A. Suri, P. Dutt, M. Sambyal, A. Ahuja, M. K. Kaul
      Pages 139-153
    9. Elizabete Catapan, Fábio Netto Moreno, Márcio Luís Busi da Silva, Michel Fleith Otuki, Rivaldo Niero, Valdir Cechinel Filho et al.
      Pages 167-177
    10. Jun Yang, Zheng-song Peng
      Pages 191-202
  3. Transgenic Approaches

    1. Front Matter
      Pages 204-204
    2. Angelina Subotić, Sladana Jevremović, Dragoljub Grubišić, Teodora Janković
      Pages 205-215
    3. G. Franklin, Margarida M. Oliveira, Alberto C. P. Dias
      Pages 217-234
    4. Karine Lièvre, Thi Lê Minh Tran, Sébastien Doerper, Alain Hehn, Paul Lacoste, Brigitte Thomasset et al.
      Pages 235-248
    5. Katarína Brunˇáková, Ján Košuth
      Pages 249-262
  4. Molecular Markers and Microsatellites

  5. Biotransformation, Bioreactors, and Metabolomics

    1. Front Matter
      Pages 304-304
    2. Brent Tisserat, Mark Berhow, Steven F. Vaughn
      Pages 313-324
    3. Takashi Asano, Hiroshi Sudo, Mami Yamazaki, Kazuki Saito
      Pages 337-345
    4. Marcelo Maraschin, Paulo Fernando Dias, Ênio Luiz Pedrotti, Hiliana Nunes, Ferreira Morais, Ana Maria Viana et al.
      Pages 347-358
  6. Altered Gravity and Biotechnology

    1. Front Matter
      Pages 372-372
    2. Lindsey K. Tuominen, Lanfang H. Levine, Mary E. Musgrave
      Pages 373-386
    3. Hemant Lata, Cassia S. Mizuno, Rita M. Moraes
      Pages 387-402
  7. Shashi B. Babbar, Neetika Walia, Amandeep Kaur, Suk Weon Kim, Myung Jin Oh
    Pages E1-E3
  8. Back Matter
    Pages 403-411

About this book


Given the vital and far-reaching applications of medicinal plant metabolites worldwide, the quality and consistency of the products as well as the very survival of various species are of the utmost importance. In Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants, expert researchers provide detailed, step-by-step protocols for the establishment of in vitro cultures of key medicinal plants, their mass multiplication in a controlled environment, and step-wise secondary metabolite analysis, genetic transformation, large-scale metabolite production in a bioreactor, and molecular markers. In addition, many of these protocols will provide a basis for much needed efforts of in vitro germplasm conservation or cryopreservation of medicinal plant species at the brink of extinction as well as efforts to protect them from the adverse impact of rapid climatic changes. As a volume in the Methods in Molecular Biology™ series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and authoritative, Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants is an ideal resource for scientists endeavoring to continue the research on this exciting natural branch of medicine.


Biotransformation Cell culture Metabolomics Micropropagation Molecular markers Plant-based medicines Seed Transgenics conservation cryopreservation gene expression genes molecular biology

Editors and affiliations

  • S. Mohan Jain
    • 1
  • Praveen K. Saxena
    • 2
  1. 1.Department of Applied BiologyUniversity of HelsinkiHelsinkiFinland
  2. 2.Department of Plant AgricultureUniversity of GuelphGuelphCanada

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2009
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60327-286-5
  • Online ISBN 978-1-60327-287-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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