Antibiotic Resistance Protocols

Second Edition

  • Stephen H. Gillespie
  • Timothy D. McHugh

Part of the Methods in Molecular Biology book series (MIMB, volume 642)

Table of contents

  1. Front Matter
    Pages i-x
  2. Epidemiology and Population Genetics

    1. Front Matter
      Pages 1-1
    2. Bambos M. Charalambous, Ndeky M. Oriyo
      Pages 13-26
  3. Genomics and Gene Expression

  4. Fitness Mutation and Physiology

  5. Detection of Resistance

    1. Front Matter
      Pages 142-142
    2. Marta Martins, Isabel Couto, Miguel Viveiros, Leonard Amaral
      Pages 143-157
    3. Miguel Viveiros, Liliana Rodrigues, Marta Martins, Isabel Couto, Gabriella Spengler, Ana Martins et al.
      Pages 159-172
    4. Mark A. Webber, Nick G. Coldham
      Pages 173-180
    5. Dimitrios Evangelopoulos, Sanjib Bhakta
      Pages 193-201
    6. Jonathan Pratten, Derren Ready
      Pages 203-215
  6. Back Matter
    Pages 225-227

About this book


Since the publication of the popular first edition, genomic methods have become more accessible, allowing antibiotic researchers to probe not only the sequence of antibiotic resistance determinants but the mechanism whereby they are expressed and regulated. That, in concert with array technology and an understanding of the importance of biofilms, has greatly expanded antibiotic resistance knowledge. In order to reflect the growing field, Antibiotic Resistance Protocols, Second Edition fully updates and builds upon its first edition with contributions from leading researchers. Beginning with chapters on epidemiology and population genetics, the book continues with sections covering genomics and gene expressions, fitness mutation and physiology, and the detection of resistance. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and up-to-date, Antibiotic Resistance Protocols, Second Edition brings together examples of a diverse range of modern techniques applied in antibiotic research in order to best aid scientists in planning their own future research.


DNA PCR biofilm gene expression genes genotypes molecular biology physiology

Editors and affiliations

  • Stephen H. Gillespie
    • 1
  • Timothy D. McHugh
    • 2
  1. 1.Div. Infection & Immunity, Research Dept. InfectionUCL Medical SchoolLondonUnited Kingdom
  2. 2.Div. Infection & Immunity, Research Dept. InfectionUCL Medical SchoolLondonUnited Kingdom

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2010
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60327-278-0
  • Online ISBN 978-1-60327-279-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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