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The Protein Protocols Handbook

  • Book
  • © 1996

Overview

Editors:
  1. John M. Walker

    1. University of Hertfordshire, Hatfield, UK

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Part of the book series: Springer Protocols Handbooks (SPH)

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Table of contents (144 protocols)

  1. Electrophoresis of Proteins and Peptides and Detection in Gels

    1. Isoelectric Focusing Under Denaturing Conditions

      • Christopher F. Thurston, Lucy F. Henley
      Pages 115-119

    2. Radioisotopic Labeling of Proteins for Polyacrylamide Gel Electrophoresis

      • Jeffrey W. Pollard
      Pages 121-126

    3. Two-Dimensional PAGE Using Carrier Ampholyte pH Gradients in the First Dimension

      • Patricia Gravel, Olivier Golaz
      Pages 127-132

    4. Two-Dimensional PAGE Using Immobilized pH Gradients

      • Graeme R. Guy, Robin J. Philp
      Pages 133-137

    5. Two-Dimensional PAGE Using Flat-Bed IEF in the First Dimension

      • Robin J. Philp
      Pages 139-142

    6. Free Zone Capillary Electrophoresis

      • David J. Begley
      Pages 143-154

    7. Capillary Isoelectric Focusing with Electro-Osmotic Flow Mobilization

      • Thomas J. Pritchett
      Pages 155-160

    8. Quantification of Radiolabeled Proteins in Polyacrylamide Gels

      • Wayne R. Springer
      Pages 161-165

    9. Quantification of Proteins by Staining in Polyacrylamide Gels

      • Bryan John Smith
      Pages 167-172

    10. Quantitation of Proteins Separated by Electrophoresis Using Coomassie Brilliant Blue

      • Ulf Neumann
      Pages 173-178

    11. Rapid Staining of Proteins in Polyacrylamide Gels with Nile Red

      • Joan-Ramon Daban, Salvador Bartolomé, Antonio Bermúdez
      Pages 179-185

    12. Zn2+-Reverse Staining of Proteins in Polyacrylamide Gels

      • Juan M. García-Segura, Mercedes Ferreras
      Pages 187-195

    13. Protein Staining with Calconcarboxylic Acid in Polyacrylamide Gels

      • Jung-Kap Choi, Hee-Youn Hong, Gyurng-Soo Yoo
      Pages 197-201

    14. Detection of Proteins and Sialoglycoproteins in Polyacrylamide Gels Using Eosin Y Stain

      • Fan Lin, Gary E. Wise
      Pages 203-205

    15. Electroelution of Proteins from Polyacrylamide Gels

      • Paul Jenö, Martin Horst
      Pages 207-214

    16. High-Performance Electrophoresis Chromatography

      • Serge Desnoyers, Sylvie Bourassa, Guy G. Poirier
      Pages 215-222

    17. Drying Polyacrylamide Gels

      • Bryan John Smith
      Pages 223-227

    18. Detection of Proteins in Polyacrylamide Gels by Silver Staining

      • Michael J. Dunn
      Pages 229-233

    19. Autoradiography and Fluorography of Acrylamide Gels

      • Antonella Circolo, Sunita Gulati
      Pages 235-242

  2. Blotting and Detection Methods

    1. Front Matter

      Pages 243-243
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Keywords

About this book

In The Protein Protocols Handbook, I have attempted to provide a cross-section of analytical techniques commonly used for proteins and peptides, thus providing a benehtop manual and guide both for those who are new to the protein chemistry laboratory and for those more established workers who wish to use a technique for the first time. We each, of course, have our own favorite, commonly used gel system, g- staining method, blotting method, and so on; I'm sure you will find yours here. H- ever, I have also described a variety of altematives for many of these techniques; though they may not be superior to the methods you commonly use, they may nev- theless be more appropriate in a particular situation. Only by knowing the range of techniques that are available to you, and the strengths and limitations of these te- niques, will you be able to choose the method that best suits your purpose.

Reviews

The Protein Protocols Handbook has arrived at the right place at the right time.-Trends in Cell Biology

Editors and Affiliations

  • University of Hertfordshire, Hatfield, UK

    John M. Walker

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