Germline Stem Cells

  • Steven X. Hou
  • Shree Ram Singh

Part of the Methods in Molecular Biology™ book series (MIMB, volume 450)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Identification and Regulation of Germline Stem Cells in Model Organisms

  3. In Vitro Culture and Applications of Germline Stem Cells

    1. Ina Dobrinski, Rahul Rathi
      Pages 139-148
    2. F. Kent Hamra, Karen M. Chapman, Zhuoru Wu, David L. Garbers
      Pages 163-179
    3. Ilaria Falciatori, Kate Lillard-Wetherell, Zhuoru Wu, F. Kent Hamra, David L. Garbers
      Pages 181-192
    4. Jason D. Heaney, Joseph H. Nadeau
      Pages 211-231
    5. Antonin Bukovsky, Satish K. Gupta, Irma Virant-Klun, Nirmala B. Upadhyaya, Pleas Copas, Stuart E. Van Meter et al.
      Pages 233-265
  4. Back Matter
    Pages 673-677

About this book


The knowledge gained by studying germline stem cells, the source of human and animal reproduction, may find immediate application in preserving endangered wildlife, managing commercial livestock, overcoming fertility problems in humans, and treating testicular and ovary tumors.  In Germline Stem Cells, leading experts explore the parameters that define germline stem cells and the mechanisms that regulate the cell behavior in order to better isolate, characterize and maintain them.  Divided into two parts, the volume begins by providing protocols for germline stem cell identification and regulation in model organisms, and concludes with detailed chapters covering current techniques involving in vitro culture and the applications of the cells.  Following the highly successful Methods in Molecular Biology™ series format, each chapter includes a brief introduction to the subject, a list of necessary equipment and reagents, step-by-step laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Germline Stem Cells is the perfect reference for scientists exploring this particular exciting and important subdivision in the fast-paced field of stem cell research.


Drosophila In vitro culture Reproductive system Stem cell behavior Vivo cell lines genes regulation tissue

Editors and affiliations

  • Steven X. Hou
    • 1
  • Shree Ram Singh
    • 1
  1. 1.National Cancer InstituteFrederickUSA

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2008
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60327-213-1
  • Online ISBN 978-1-60327-214-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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