Mesenchymal Stem Cells

Methods and Protocols

  • Darwin J. Prockop
  • Bruce A. Bunnell
  • Donald G. Phinney

Part of the Methods in Molecular Biology™ book series (MIMB, volume 449)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Isolation of Human Multipotential Stromal Cells from Bone Marrow and Adipose Tissue

    1. Margaret Wolfe, Radhika Pochampally, William Swaney, Roxanne L. Reger
      Pages 3-25
    2. Mark F. Pittenger
      Pages 27-44
    3. Stan Gronthos, Andrew C. W. Zannettino
      Pages 45-57
    4. John K. Fraser, Min Zhu, Isabella Wulur, Zeni Alfonso
      Pages 59-67
    5. Severine G. Dubois, Elizabeth Z. Floyd, Sanjin Zvonic, Gail Kilroy, Xiying Wu, Stacy Carling et al.
      Pages 69-79
  3. Manipulation of Human Multipotential Stromal Cells

    1. Radhika Pochampally
      Pages 83-91
    2. Roxanne L. Reger, Alan H. Tucker, Margaret R. Wolfe
      Pages 93-107
    3. Joni Ylőstalo, Radhika Pochampally, Darwin J. Prockop
      Pages 133-151
    4. Reza Izadpanah, Bruce A. Bunnell
      Pages 153-167
  4. Isolation and Characterization of Murine Multipotential Stromal Cells from Bone Marrow

  5. Back Matter
    Pages 187-192

About this book


For over forty years, mesenchymal stem cells (MSCs) have been scrutinized and studied, garnering much attention due to their broad therapeutic efficacy. In Mesenchymal Stem Cells: Methods and Protocols, leaders in the field were assembled to contribute detailed methodologies for the isolation and characterization of human and rodent MSCs. Recently, these vital cells have shown therapeutic benefits in the treatment of myocardial infarction, stroke, lung diseases, spinal cord injury and other neurological disorders, thus promising a boundless future in their study. Written to the specifications of the highly successful Methods in Molecular Biology™ series format, each chapter contains step-by-step laboratory protocols, a list of the necessary materials, and a Notes section with tips on troubleshooting and avoiding known pitfalls.

Cutting edge and easy to use, Mesenchymal Stem Cells: Methods and Protocols is the perfect resource for scientists attempting to pursue this important and ever-developing field of research.


MSCs Marrow stromal cells Microarray Multipotent mesenchymal stromal cells gene expression genes tissue

Editors and affiliations

  • Darwin J. Prockop
    • 1
  • Bruce A. Bunnell
    • 1
  • Donald G. Phinney
    • 1
  1. 1.Center for Gene TherapyTulane University Health Sciences CenterNew OrleansLA

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2008
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-771-6
  • Online ISBN 978-1-60327-169-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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