© 2009


Methods and Protocols, Volume 1: Isolation, Characterization, and Interactions

  • Martha R.J. Clokie
  • Andrew M. Kropinski

Part of the Methods in Molecular Biology™ book series (MIMB, volume 501)

Table of contents

  1. Front Matter
    Pages i-xxii
  2. Bacteriophage Isolation

    1. Front Matter
      Pages 1-1
    2. K. Eric Wommack, Kurt E. Williamson, Rebekah R. Helton, Shellie R. Bench, Danielle M. Winget
      Pages 3-14
    3. Rohan Van Twest, Andrew M. Kropinski
      Pages 15-21
    4. Ra’l R. Raya, Elvira M. H’bert
      Pages 23-32
    5. Jennifer Fulton, Trevor Douglas, and Mark Young
      Pages 43-54
    6. Philip Serwer, Shirley J. Hayes, Julie A. Thomas, Borries Demeler, Stephen C. Hardies
      Pages 55-66
  3. Bacteriophage Characterization

    1. Front Matter
      Pages 67-67
    2. Andrew M. Kropinski, Amanda Mazzocco, Thomas E. Waddell, Erika Lingohr, Roger P. Johnson
      Pages 69-76
    3. Amanda Mazzocco, Thomas E. Waddell, Erika Lingohr, Roger P. Johnson
      Pages 77-80
    4. Amanda Mazzocco, Thomas E. Waddell, Erika Lingohr, Roger P. Johnson
      Pages 81-85
    5. Alice C. Ortmann, Curtis A. Suttle
      Pages 87-95
    6. Corina P.D. Brussaard
      Pages 97-111
    7. Hans-W. Ackermann
      Pages 113-126
    8. Hans-W. Ackermann
      Pages 127-140
    9. Elizabeth Kutter
      Pages 141-149
    10. Stephen T. Abedon, John Yin
      Pages 161-174
    11. Paul Hyman, Stephen T. Abedon
      Pages 175-202
    12. Louis-Charles Fortier, Sylvain Moineau
      Pages 203-219
  4. Bacteriophage-Host Interactions

    1. Front Matter
      Pages 220-220
    2. Robert Villafane
      Pages 223-237
    3. Lynn C. Thomason, Amos B. Oppenheim, Donald L. Court
      Pages 239-251
    4. Jonathan Livny, Christopher N. LaRock, David I. Friedman
      Pages 253-265
    5. Anne Thierauf, Gerardo Perez, and Stanley Maloy
      Pages 267-286
    6. Thomas E. Waddell, Kristyn Franklin, Amanda Mazzocco, Roger P. Johnson
      Pages 287-292
    7. Thomas E. Waddell, Kristyn Franklin, Amanda Mazzocco, Andrew M. Kropinski, Roger P. Johnson
      Pages 293-303
  5. Back Matter
    Pages 305-307

About this book


Ranging from the evolution of pathogenicity to oceanic carbon cycling, the many and varied roles that bacteriophages play in microbial ecology and evolution have inspired increased interest within the scientific community. Bacteriophages: Methods and Protocols pulls together the vast body of knowledge and expertise from top international bacteriophage researchers to provide both classical and state-of-the-art molecular techniques.  With its well-organized modular design, Volume 1: Isolation, Characterization, and Interactions examines a multitude of topics, including the isolation of phages, morphological and molecular characterization, and interaction with bacteria.  Written in the highly successful Methods in Molecular Biology™ series format, chapters consist of brief introductions to the subject, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and a Notes section which details tips on troubleshooting and avoiding known pitfalls.

Thorough and cutting-edge, Bacteriophages: Methods and Protocols is a valuable reference for experienced bacteriophage researchers as well as an easily accessible introduction for newcomers to the subject.


Bacteria Microbial ecology Microbial evolution Morphology Pathogenicity Termination electron microscopy molecular biology

Editors and affiliations

  • Martha R.J. Clokie
    • 1
  • Andrew M. Kropinski
    • 2
  1. 1.University of LeicesterUK
  2. 2.Public Health Agency of CanadaGuelphCanada

Bibliographic information

  • Book Title Bacteriophages
  • Book Subtitle Methods and Protocols, Volume 1: Isolation, Characterization, and Interactions
  • Editors Martha R. J. Clokie
    Andrew Kropinski
  • Series Title Methods in Molecular Biology™
  • DOI
  • Copyright Information Humana Press 2009
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-58829-682-5
  • Softcover ISBN 978-1-61737-715-0
  • eBook ISBN 978-1-60327-164-6
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 1
  • Number of Pages XXII, 308
  • Number of Illustrations 41 b/w illustrations, 0 illustrations in colour
  • Topics Biochemistry, general
    Laboratory Medicine
  • Buy this book on publisher's site
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From the reviews:

"This book apart from the numerous texts recently published in the area of bacteriophage biology is the focus on the methodology used in this field. … This volume focuses largely on the classical protocols - as described by the editors … . This book should prove to be a valuable resource to even the experienced phage biologist and an essential reference book for postgraduate students and scientists new to the field of phage biology." (Sophie Foley, Microbiology Today, July, 2009)