Expressed Sequence Tags (ESTs)

Generation and Analysis

  • John Parkinson

Part of the Methods in Molecular Biology book series (MIMB, volume 533)

Table of contents

  1. Front Matter
    Pages i-x
  2. John Parkinson, Mark Blaxter
    Pages 1-12
  3. Sandra W. Clifton, Makedonka Mitreva
    Pages 13-32
  4. Naiara Rodríguez-Ezpeleta, Shona Teijeiro, Lise Forget, Gertraud Burger, B. Franz Lang
    Pages 33-47
  5. Motoaki Seki, Asako Kamiya, Piero Carninci, Yoshihide Hayashizaki, Kazuo Shinozaki
    Pages 49-68
  6. Masako Harada, Yoshihide Hayashizaki
    Pages 69-107
  7. Marcelo Bento Soares, Maria de Fatima Bonaldo, Jeremiah D. Hackett, Debashish Bhattacharya
    Pages 109-123
  8. Cecilia Fernández, Rick M. Maizels
    Pages 125-151
  9. Makedonka Mitreva, Elaine R. Mardis
    Pages 153-187
  10. Ralf Schmid, Mark Blaxter
    Pages 189-220
  11. James Wasmuth, Mark Blaxter
    Pages 221-239
  12. Yao-Qing Shen, Emmet O’Brien, Liisa Koski, B. Franz Lang, Gertraud Burger
    Pages 241-256
  13. José M. Peregrín-Alvarez, John Parkinson
    Pages 257-276
  14. Edward Susko, Andrew J. Roger
    Pages 277-287
  15. Weizhong Li, Lisa Olohan, Daryl Williams, Margaret Hughes, Andrew Gracey, Andrew Cossins
    Pages 289-309
  16. Sandro José de Souza
    Pages 311-324
  17. Back Matter
    Pages 325-331

About this book


Over the past two decades, expressed sequence tags (ESTs - single pass reads from randomly selected cDNAs), have proven to be a remarkably cost-effective route for the purposes of gene discovery. Gaining in popularity, millions of ESTs have now been generated for over a thousand different species. In Expressed Sequence Tags (ESTs): Generation and Analysis, leading experts in the field introduce the reader to many of the fundamental concepts underlying the generation and analysis of ESTs through readily accessible and affordable sequencing technologies. The volume focuses on various methods used to generate, process and analyze EST datasets, while also exploring the use of EST technology for other purposes such as expression profiling, analysis of alternative transcripts, and phylogenomics. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and programs, step-by-step, readily reproducible protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and easy to use, Expressed Sequence Tags (ESTs): Generation and Analysis serves as an ideal reference for scientists continuing the vital investigation of genes and the genome.


Alternative transcripts Bur Expression profiling Gene discovery Genetic diversity Microarray Phylogenomics Sequence data Translation cDNA clones genes molecular biology

Editors and affiliations

  • John Parkinson

There are no affiliations available

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2009
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-759-4
  • Online ISBN 978-1-60327-136-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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