Protocols for In Vitro Propagation of Ornamental Plants

  • S. Mohan Jain
  • Sergio J. Ochatt

Part of the Methods in Molecular Biology book series (MIMB, volume 589)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Protocols for In Vitro Propa gation of Ornamental Plants

    1. Front Matter
      Pages 1-1
    2. Oropeza Maira, Mejías Alexander, Teresa Edith Vargas
      Pages 3-14
    3. Duong Tan Nhut, Nguyen Thanh Hai, Mai Xuan Phan
      Pages 15-20
    4. M. Firoz Alam, Pinaki Sinha, M. Lokman Hakim
      Pages 21-28
    5. Purnima Tyagi, P. K. Sharma, S. L. Kothari
      Pages 39-46
    6. Miguel Pedro Guerra, Lirio Luiz Dal Vesco
      Pages 47-66
    7. Marcos Castellanos, J. Brian Power, Michael R. Davey
      Pages 67-75
    8. Pinaki Sinha, M. Firoz Alam, M. Lokman Hakim
      Pages 77-85
    9. Chalermsri Nontaswatsri, Seiichi Fukai
      Pages 87-96
    10. Jose L. Casas, Enrique Olmos, Abel Piqueras
      Pages 109-116
    11. Toshinari Godo, Yuanxue Lu, Masahiro Mii
      Pages 127-139
    12. Tom Eeckhaut, Kristien Janssens, Ellen De Keyser, Jan De Riek
      Pages 141-152
    13. Luca Pipino, Luca Braglia, Annalisa Giovannini, Giancarlo Fascella, Antonio Mercuri
      Pages 153-162
    14. Pratap Kumar Pati, Navtej Kaur, Madhu Sharma, Paramvir Singh Ahuja
      Pages 163-176
    15. D. Nencheva
      Pages 177-185
    16. Noëlle Dorion, Hatem Ben Jouira, Anthony Gallard, Anber Hassanein, Mazen Nassour, Agnès Grapin
      Pages 197-211
    17. Alma Angélica Del Villar-Martínez, Pablo Emilio Vanegas-Espinoza, Octavio Paredes-López
      Pages 213-221
    18. Chui Eng Wong, Prem L. Bhalla
      Pages 235-241
    19. Małgorzata Podwyszyńska, Dariusz Sochacki
      Pages 243-256
    20. Barbara Ruffoni, Carlo Mascarello, Marco Savona
      Pages 257-269
    21. M. L. Mayor, G. Nestares, T. Vega, R. Zorzoli, L. A. Picardi
      Pages 271-280
  3. Section B Protocols for In Vitro Propa gation of Ornamental Plants

    1. Front Matter
      Pages 292-292
    2. Sergio J. Ochatt, C. Conreux, L. Jacas
      Pages 293-301
    3. Elif Aylin Ozudogru, Alberto Previati, Maurizio Lambardi
      Pages 303-324
    4. Ellen De Keyser, Valentina Scariot, Nobuo Kobayashi, Takashi Handa, Jan De Riek
      Pages 349-364
    5. Johan Van Huylenbroeck
      Pages 365-376
  4. Back Matter
    Pages 393-400

About this book


While ornamental plants are produced mainly for their aesthetic value, the propagation and improvement of quality attributes such as leaf types, flower colour and fragrance, longevity and form, plant shape and architecture, and the creation of novel variation are important economic goals for the expanding ornamental industry.  In Protocols for In Vitro Propagation of Ornamental Plants, leading researchers in the field compile step-wise protocols for rapid plant multiplication and in vitro storage of major commercially viable ornamental plants.  Divided into two sections, Section A contains chapters mainly on micropropagation of cut and pot flowers with detailed protocols involving in vitro culture-explants, medium preparation, detailed medium table, shoot initiation and proliferation, root induction, in vitro plant hardening, and field transfer, and Section B delves into reviews on topics such as in vitro production of sweet peas, the status of transgenics in ornamental plants, in vitro conservation, the status of floriculture in Europe, azalea phylogeny, and thin cell layers.  As part of the highly successful Methods in Molecular Biology™ series, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Comprehensive and clear, Protocols for In Vitro Propagation of Ornamental Plants presents key techniques that will be of great use to floriculturists, researchers, commercial companies, biotechnologists, and students dealing with ornamental plants.


Floriculture Large-scale plant multiplication Micropropagation Ornamental cultivars Ornamental plant Proliferation Transgenesis Tree Vine genetic engineering phylogeny quality

Editors and affiliations

  • S. Mohan Jain
    • 1
  • Sergio J. Ochatt
    • 2
  1. 1.Department of Applied BiologyUniversity of HelsinkiHelsinkiFinland
  2. 2.UMRLEG, PCMVINRADijon CXFrance

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2010
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60327-390-9
  • Online ISBN 978-1-60327-114-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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