2D PAGE: Sample Preparation and Fractionation

  • Anton Posch

Part of the Methods in Molecular Biology™ book series (MIMB, volume 424)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Sample Preparation Basics

  3. Protein Labeling Techniques

    1. Front Matter
      Pages 71-71
    2. Reiner Westermeier, Burghardt Scheibe
      Pages 73-85
    3. Pier Giorgio Righetti, Roberto Sebastiano, Attilio Citterio
      Pages 87-99
    4. s Gruhler, Irina Kratchmarova
      Pages 101-111
    5. Josef Kellermann
      Pages 113-123
    6. Hélian Boucherie, Aurélie Massoni, Christelle Monribot-Espagne
      Pages 125-135
  4. Fractionation of Proteins by Chemical Reagents and Chromatography

  5. Fractionation of Proteins by Electrophoresis Methods

    1. Front Matter
      Pages 223-223
    2. Julie Hey, Anton Posch, Andrew Cohen, Ning Liu, Adrianna Harbers
      Pages 225-239
    3. Mee-Jung Han, David W Speicher
      Pages 241-256
    4. Angelika Görg, Carsten Lück, Walter Weiss
      Pages 277-286
    5. Robert Wildgruber, Jizu Yi, Mikkel Nissum, Christoph Eckerskorn, Gerhard Weber
      Pages 287-300
    6. Michael Fountoulakis, Ploumisti Dimitraki
      Pages 301-313
  6. Enrichment Strategies for Organelles, Multiprotein Complexes, and Specific Protein Classes

    1. Front Matter
      Pages 315-315
    2. MarianaEÇaGuimarães Araùjo, Lukas Alfons Hube, Taras Stasyk
      Pages 317-331
    3. Hans Zischka, Josef Lichtmannegger, Nora Jägemann, Luise Jennen, Daniela Hamöller, Evamaria Huber et al.
      Pages 333-348
    4. Barbara Kaboord, Maria Perr
      Pages 349-364
    5. Lawrence G. Puente, Lynn A. Megeney
      Pages 365-372
    6. Yehia Mechref, Milan Madera, Milos V Novotny
      Pages 373-396
    7. Henrik Everberg, Niklas Gustavasson, Folke Tjerned
      Pages 403-412
    8. Veronika Reisinger, Lutz A. Eichacker
      Pages 423-431
    9. Heidi S. Erickson, John W. Gillespie, Michael R. Emmert-Buck
      Pages 433-448
  7. Back Matter
    Pages 449-459

About this book


The complex nature of proteins often requires a multitude of sample preparation options. In 2D PAGE: Sample Preparation and Fractionation, Volumes 1 & 2, Dr. Anton Posch presents a broad coverage of the principles and recent developments of sample preparation and fractionation tools in Expression Proteomics and specifically for two-dimensional electrophoresis (2-DE). Detailed laboratory methods describe the process of sample fractionation used to reduce the complexity of the protein sample and the methods of 2-DE analysis and a broad variety of gel-free mass spectrometry-based technologies to detect previously undetectable proteins. Vital information regarding protein isolation and preparation for reproducible proteome analysis previously overlooked by scientists has been clearly laid out in this pioneering two-volume set. Volume 1 focuses on the basics of sample preparation and protein labeling techniques with an emphasis on fractionation by chemical reagents, chromatography, and electrophoresis methods. The inclusion of meticulous enrichment strategies further aids the protein researcher.


Invaluable for students of Biochemistry, Biomedicine, Biology and Genomics and essential for the practicing scientists, 2D PAGE: Sample Preparation and Fractionation, Volumes 1 & 2 delve into the study of proteins with comprehensive and in depth methods and protocols to provide a greater understanding of this intricate and complicated field.


Amino acid Escherichia coli Lipid Organelle Polyacrylamid-Gelelektrophorese Proteomics biochemistry biomedicine enzymes glycoprotein membrane proteins polymer protein protein complexes proteins

Editors and affiliations

  • Anton Posch
    • 1
  1. 1.Bio-Rad Laboratories GmbHGermany

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2008
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-722-8
  • Online ISBN 978-1-60327-064-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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