Structural Proteomics

High-Throughput Methods

  • Bostjan Kobe
  • Mitchell Guss
  • Thomas Huber

Part of the Methods in Molecular Biology™ book series (MIMB, volume 426)

Table of contents

  1. Front Matter
    Pages i-xxvi
  2. Protein Target Selection, Bioinformatic Approaches, and Data Management

    1. Russell L. Marsden, Christine A. Orengo
      Pages 3-25
    2. Gautier Robin, Nathan P. Cowieson, Gregor Guncar, Jade K. Forwood, Pawel Listwan, David A. Hume et al.
      Pages 27-35
    3. Sabrina Haquin, Eric Oeuillet, Anne Pajon, Mark Harris, Alwyn T. Jones, Herman van Tilbeurgh et al.
      Pages 49-79
    4. Shuchismita Dutta, Kyle Burkhardt, Ganesh J. Swaminathan, Takashi Kosada, Kim Henrick, Haruki Nakamura et al.
      Pages 81-101
    5. Zsuzsanna Dosztányi, Peter Tompa
      Pages 103-115
    6. Helgi Ingolfsson, Golan Yona
      Pages 117-143
    7. Narayanan Eswar, David Eramian, Ben Webb, Min-Yi Shen, Andrej Sali
      Pages 145-159
  3. Protein Production

    1. Volker Sievert, Asgar Ergin, Konrad Büssow
      Pages 163-173
    2. Didier Busso, Matthieu Stierlé, Jean-Claude Thierry, Dino Moras
      Pages 175-186
    3. Kyoung Hoon Kim, Jin Kuk Yang, Geoffrey S. Waldo, Thomas C. Terwilliger, Se Won Suh
      Pages 187-195
    4. Yunjia Chen, Shihong Qiu, Chi-Hao Luan, Ming Luo
      Pages 209-220
    5. Opher Gileadi, Nicola A. Burgess-Brown, Steve M. Colebrook, Georgina Berridge, Pavel Savitsky, Carol E. A. Smee et al.
      Pages 221-246
    6. Margit A. Apponyi, Kiyoshi Ozawa, Nicholas E. Dixon, Gottfried Otting
      Pages 257-268
    7. Nathan P. Cowieson, Beth Wensley, Gautier Robin, Gregor Guncar, Jade Forwood, David A. Hume et al.
      Pages 269-275
  4. Biophysical and Functional Characterization of Proteins

    1. Michael Proudfoot, Ekaterina Kuznetsova, Stephen A. Sanders, Claudio F. Gonzalez, Greg Brown, Aled M. Edwards et al.
      Pages 331-341
  5. Structural Characterization of Proteins

    1. Marc Pusey, Elizabeth Forsythe, Aniruddha Achari
      Pages 377-385
    2. Anil S. Thakur, Janet Newman, Jennifer L. Martin, Bostjan Kobe
      Pages 403-409
    3. Ran Meged, Orly Dym, Joel L. Sussman
      Pages 411-418
    4. Peter H. Zwart, Pavel V. Afonine, Ralf W. Grosse-Kunstleve, Li-Wei Hung, Thomas R. Ioerger, Airlie J. McCoy et al.
      Pages 419-435
    5. Dmitri Mouradov, Gordon King, Ian L. Ross, Jade K. Forwood, David A. Hume, Andrea Sinz et al.
      Pages 459-474
  6. Structural Proteomics Initiatives Overviews

    1. Sung-Hou Kim, Dong-Hae Shin, Rosalind Kim, Paul Adams, John-Marc Chandonia
      Pages 475-496
    2. Erkang Fan, David Baker, Stanley Fields, Michael H. Gelb, Frederick S. Buckner, Wesley C. Van Voorhis et al.
      Pages 497-513
    3. Alexey Bochkarev, Wolfram Tempel
      Pages 515-521
    4. Didier Busso, Jean-Claude Thierry, Dino Moras
      Pages 523-536
    5. Michael J. Sauder, Marc E. Rutter, Kevin Bain, Isabelle Rooney, Tarun Gheyi, Shane Atwell et al.
      Pages 561-575
    6. Weining Meng, Jade K. Forwood, Gregor Guncar, Gautier Robin, Nathan P. Cowieson, Pawel Listwan et al.
      Pages 577-587
    7. Andrew Robinson, Amy P. Guilfoyle, Visaahini Sureshan, Michael Howell, Stephen J. Harrop, Yan Boucher et al.
      Pages 589-595
  7. Back Matter
    Pages 597-601

About this book


Structural genomics is a newly emerging field that has arisen following the successful footsteps of the major sequencing efforts generally bundled under the heading "genomics". In Structural Proteomics: High Throughput Methods, readers are provided with a current view of all aspects of the 'pipeline' that takes protein targets to structures and how these have been optimized. This volume includes chapters describing in-depth the individual steps in the Structural Genomics pipeline, as well as overviews of individual Structural Genomics initiatives. Topics include Target Selection for structural genomics, Protein structure modeling, High-throughput cloning, Mass Spectrometry modeling. Structural Proteomics: High Throughput Methods, encourages its readers to access further details on the methodologies in on-line resources as well as in cited literature making it an invaluable resource. Most methods in this important book are as amenable to small laboratories as to large consortia, and do not require major investments in facilities. This essential volume provides insight into the diversity of approaches adopted by different laboratories and proves that 'high throughput' is really the defining characteristic of structural genomics.


Annotation Escherichia coli High-throughput cloning Protein structure modeling Proteomics Structural genomics Termination biology enzymes genes genome membrane proteins protein complexes protein structure proteins

Editors and affiliations

  • Bostjan Kobe
    • 1
  • Mitchell Guss
    • 2
  • Thomas Huber
    • 3
  1. 1.School of Molecular and Microbial SciencesThe University of QueenslandBrisbane, QueenslandAustralia
  2. 2.School of Molecular and Microbial BiosciencesUniversity of SydneySydneyAustralia
  3. 3.School of Molecular and Microbial Sciences and Australian Institute for Bioengineering and NanotechnologyThe University of QueenslandBrisbaneAustralia

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2008
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-809-6
  • Online ISBN 978-1-60327-058-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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