Free Radicals and Antioxidant Protocols

  • Rao M. Uppu
  • Subramanyam N. Murthy
  • William A.  Pryor
  • Narasimham L. Parinandi

Part of the Methods in Molecular Biology book series (MIMB, volume 610)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Reactive Oxygen and Nitrogen Species

    1. Front Matter
      Pages 1-1
    2. Deepti S. Vikram, Brian K. Rivera, Periannan Kuppusamy
      Pages 3-27
    3. Enika Nagababu, Joseph M. Rifkind
      Pages 41-49
    4. K. Sathishkumar, Xueli Gao, Achuthan C. Raghavamenon, Subramanyam N. Murthy, Philip J. Kadowitz, Rao M. Uppu
      Pages 51-61
  3. Natural and Synthetic Antioxidants

    1. Front Matter
      Pages 63-63
    2. Osamu Cynshi, Kunio Tamura, Etsuo Niki
      Pages 91-107
    3. Adriana R. Pohlmann, Scheila Rezende Schaffazick, Tânia B. Creczynski-Pasa, Sílvia S. Guterres
      Pages 109-121
    4. Gang Liu, Ping Men, George Perry, Mark A. Smith
      Pages 123-144
    5. Eric Simone, Thomas Dziubla, Vladimir Shuvaev, Vladimir R. Muzykantov
      Pages 145-164
    6. Enika Nagababu, Joseph M. Rifkind, Sesikeran Boindala, Lakshmaiah Nakka
      Pages 165-180
  4. Cellular Oxidative Stress

    1. Front Matter
      Pages 181-181
    2. Michelle A. Kline, E. S. O’Connor Butler, Adam Hinzey, Sean Sliman, Sainath R. Kotha, Clay B. Marsh et al.
      Pages 201-211
    3. W. Deng, T.J. Bivalacqua, H.C. Champion, W.J. Hellstrom, Subramanyam N. Murthy, Philip J. Kadowitz
      Pages 213-227
    4. Muthuswamy Balasubramanyam, Antonysunil Adaikalakoteswari, Zaheer Sameermahmood, Viswanathan Mohan
      Pages 245-261
    5. K. Sathishkumar, Achuthan C. Raghavamenon, Karunakaran Ganeshkumar, Rameshwari Telaprolu, Narasimham L. Parinandi, Rao M. Uppu
      Pages 263-284
    6. Vittorio Calabrese, Carolin Cornelius, Cesare Mancuso, Riccardo Lentile, A.M. Giuffrida Stella, D. Allan Butterfield
      Pages 285-308
    7. W. Deng, T.J. Bivalacqua, H.C. Champion, W.J. Hellstrom, Subramanyam N. Murthy, Philip J. Kadowitz
      Pages 309-321
  5. DNA Oxidation, Oxidative Lipidomics, and Biomarkers

    1. Front Matter
      Pages 323-323
    2. Mark D. Evans, Rajinder Singh, Vilas Mistry, Peter B. Farmer, Marcus S. Cooke
      Pages 341-351
    3. Vladimir A. Tyurin, Yulia Y. Tyurina, Vladimir B. Ritov, Andriy Lysytsya, Andrew A. Amoscato, Patrick M. Kochanek et al.
      Pages 353-374
    4. E. Susan O’Connor Butler, Jessica N. Mazerik, Jason P. Cruff, Shariq I. Sherwani, Barbara K. Weis, Clay B. Marsh et al.
      Pages 387-401
    5. Sampath Parthasarathy, Achuthan Raghavamenon, Mahdi Omar Garelnabi, Nalini Santanam
      Pages 403-417
    6. Paula I. Moreira, Lawrence M. Sayre, Xiongwei Zhu, Akihiko Nunomura, Mark A. Smith, George Perry
      Pages 419-434
    7. Denis M. Callewaert, Charles Sloan
      Pages 435-449
    8. Victor Ma, Heather Lord, Melissa Morley, Janusz Pawliszyn
      Pages 451-468
  6. Back Matter
    Pages 469-480

About this book


In recent years there has been a growing demand to achieve more rapid and accurate detection of RONs (reactive oxygen and nitrogen species), which affect normal physiological processes and pathological conditions, and also of redox stress. In Free Radicals and Antioxidant Protocols, Second Edition, world-renowned researchers respond to this need, considering the most recent analytical and technological aspects of the detection and determination of RONs, and of oxidative, nitrative, nitrosative, and redox stress in biological systems in vivo and in vitro. Chapters include protocols to establish biomarkers for the oxidative stress-induced genetic damage in cellular and animal models, provide novel methods and technologies for analyzing peroxidized lipids, describe techniques for gene therapy, and present methods of EPR imaging of free radicals and oxygen in vivo. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Comprehensive and user-friendly, Free Radicals and Antioxidant Protocols, Second Edition, is an essential guide for the novice and expert researcher alike.


DNA EPR imaging Electron paramagnetic resonance microscopy Gene Transfer Lipid Lipid peroxidation Oxidation RONs Reactive oxygen and nitrogen species Redox stress enzymes gene expression

Editors and affiliations

  • Rao M. Uppu
    • 1
  • Subramanyam N. Murthy
    • 2
  • William A.  Pryor
    • 3
  • Narasimham L. Parinandi
    • 4
  1. 1.A& M CollegeSouthern University &Baton RougeU.S.A.
  2. 2.School of MedicineTulane UniversityNew OrleansU.S.A.
  3. 3.Biodynamics Inst.Louisiana State UniversityBaton RougeU.S.A.
  4. 4.Dorothy M. Davis Heart &Ohio State UniversityColumbusU.S.A.

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2010
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-710-5
  • Online ISBN 978-1-60327-029-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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