Methods and Protocols, Second Edition

  • Peter Erhardt
  • Ambrus Toth

Part of the Methods in Molecular Biology book series (MIMB, volume 559)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Detection of Apoptosis

    1. Front Matter
      Pages 1-1
    2. Donald Wlodkowic, Joanna Skommer, Zbigniew Darzynkiewicz
      Pages 19-32
    3. Stephen W.G. Tait, Lisa Bouchier-Hayes, Andrew Oberst, Samuel Connell, Douglas R. Green
      Pages 33-48
    4. Eva Csizmadia, Vilmos Csizmadia
      Pages 49-63
    5. Dhyan Chandra, Dean G. Tang
      Pages 65-75
  3. Detection of Non-Apoptotic Cell Death

    1. Front Matter
      Pages 78-78
    2. Benchun Miao, Alexei Degterev
      Pages 79-93
    3. Narasimman Gurusamy, Dipak K. Das
      Pages 95-103
  4. Modifications of Apoptotic Proteins during Apoptosis

    1. Front Matter
      Pages 106-106
    2. Zoltán Nemes, László Fésüs
      Pages 107-116
    3. Neelam Azad, Anand Krishnan V. Iyer, Yon Rojanasakul
      Pages 117-130
  5. Analysis of the Function of Major Regulators of Apoptosis/Cell Survival

    1. Front Matter
      Pages 142-142
    2. Darren C. Phillips, Sean P. Garrison, John R. Jeffers, Gerard P. Zambetti
      Pages 143-159
    3. Andrew Fribley, Kezhong Zhang, Randal J. Kaufman
      Pages 191-204
    4. Zoltan Derdak, Tamako A. Garcia, Gyorgy Baffy
      Pages 205-217
    5. Riccardo Spizzo, George A. Calin
      Pages 219-245
    6. Kathleen A. McPhillips, Lars-Peter Erwig
      Pages 247-256
  6. Analysis of Apoptosis in Different Organs

    1. Front Matter
      Pages 258-258
    2. Lin Lin, Carlos Penaloza, Yixia Ye, Richard A. Lockshin, Zahra Zakeri
      Pages 259-272
    3. Youngsoo Lee, Peter J. McKinnon
      Pages 273-282
    4. Adel Mandl, Ambrus Toth, Peter Erhardt
      Pages 293-311
    5. Lawrence M. Schwartz, Zhengliang Gao, Christine Brown, Sangram S. Parelkar, Honor Glenn
      Pages 313-332
  7. Analysis of Apoptosis in Model Organisms

    1. Front Matter
      Pages 334-334
    2. Xinchen Teng, J. Marie Hardwick
      Pages 335-342
    3. Kimberly McCall, Jeanne S. Peterson, Tracy L. Pritchett
      Pages 343-356
    4. Christopher R. Cotter, John A. Blaho
      Pages 371-387
  8. Back Matter
    Pages 389-399

About this book


The ability to detect and quantify apoptosis, to understand its biochemistry, and to identify its regulatory genes and proteins is crucial to biomedical research. In Apoptosis: Methods and Protocols, Second Edition, expert researchers describe the techniques to best investigate the critical steps involved in the apoptotic process. Presented from several different research perspectives, the volume contains sections covering detection of apoptosis, detection of non-apoptotic cell death, modifications of apoptotic proteins during apoptosis, the analysis of its major regulators, as well as analysis of apoptosis in different organs and in model organisms. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Apoptosis: Methods and Protocols, Second Edition constitutes a key technical reference to the significant methodologies used in the field, and offers beginners and experienced researchers powerful tools to illuminate the phenomena of programmed cell death.


Apoptotic cell death Autophagy Flow cytometry Necroptosis Posttranslational modifications genes molecular biology

Editors and affiliations

  • Peter Erhardt
    • 1
  • Ambrus Toth
    • 2
  1. 1.Boston Biomedical Research InstituteWatertownU.S.A.
  2. 2.Boston Biomedical Research InstituteWatertownU.S.A.

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2009
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60327-016-8
  • Online ISBN 978-1-60327-017-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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