Proteases and Cancer

Methods and Protocols

  • Thomas H. Bugge
  • Toni M. Antalis

Part of the Methods in Molecular Biology™ book series (MIMB, volume 539)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Nesrine I. Affara, Pauline Andreu, Lisa M. Coussens
    Pages 1-32
  3. Gonzalo R. Ordóñez, Xose S. Puente
, Víctor Quesada, Carlos López-Otín
    Pages 33-47
  4. Kamiar Moin, Donald Schwartz, Stefanie R. Mullins, Bonnie F. Sloane
    Pages 49-57
  5. Boris Ratnikov, Piotr Cieplak, Jeffrey W. Smith
    Pages 93-114
  6. John P. Hobson, , Shihui Liu, Stephen H. Leppla, Thomas H. Bugge
    Pages 115-129
  7. Shihui Liu, Thomas H. Bugge, Arthur E. Frankel, Stephen H. Leppla
    Pages 175-190
  8. Pappanaicken R. Kumaresan, Juntao Luo, Kit S. Lam
    Pages 191-211
  9. Back Matter
    Pages 213-221

About this book


Proteases decisively contribute to cancer development and promotion by regulating the activities of growth factors/cytokines and signaling receptors, as well as the composition of the extracellular matrix, thereby suppressing cell death pathways and activating cell survival pathways. In Proteases and Cancer: Methods and Protocols, expert researchers bring together a wide range of current, complimentary techniques that have been developed for the specific detection and analysis of proteases and their activities in cancer biology. The volume covers vital topics including the application of proteomics technologies for the detection of protease expression in tumors, imaging proteases by activity profiling, proteomics technologies for the identification of biological substrates, detection of cell surface proteolysis, imaging of protease activity, the use of transgenic mice to determine protease function in tumor initiation and progression, and the development of anti-protease therapies for cancer. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easy to use, Proteases and Cancer: Methods and Protocols is an ideal guide for scientists who wish to pursue the study of this important branch of cancer research and the development of unique innovative technologies to aid in that study.


Cancer development Protein cleavage Proteolytic enzymes Tumor cells imaging tissue

Editors and affiliations

  • Thomas H. Bugge
  • Toni M. Antalis

There are no affiliations available

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2009
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60327-002-1
  • Online ISBN 978-1-60327-003-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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