© 2008

Affinity Chromatography

Methods and Protocols

  • Michael Zachariou

Part of the Methods in Molecular Biology™ book series (MIMB, volume 421)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Affinity Chromatography

    1. Front Matter
      Pages 1-1
    2. Ana Cecília A. Roque, Christopher R. Lowe
      Pages 1-23
  3. Various Modes of Affinity Chromatography

    1. Front Matter
      Pages 23-23
    2. Adam Charlton, Michael Zachariou
      Pages 25-36
    3. Ashok Kumar, Igor Yu. Galaev, Bo Mattiasson
      Pages 37-52
    4. Stuart R. Gallant, Vish Koppaka, Nick Zecherle
      Pages 53-60
    5. Stuart R. Gallant, Vish Koppaka, Nick Zecherle
      Pages 61-70
  4. Affinity Chromatography Using Purification Tags

    1. Front Matter
      Pages 91-91
    2. Ana Cecília A. Roque, Christopher R. Lowe
      Pages 93-110
    3. Joanne L. Casey, Andrew M. Coley, Michael Foley
      Pages 111-124
    4. Becky Godat, Laurie Engel, Natalie A. Betz, Tonny M. Johnson
      Pages 151-168
    5. Leonard K Pattenden, Walter G Thomas
      Pages 169-190
    6. Marjeta Urh, Danette Hartzell, Jacqui Mendez, Dieter H. Klaubert, Keith Wood
      Pages 191-210
    7. Adam Charlton
      Pages 211-228
    8. José Arnau, Conni Lauritzen, Gitte Ebert Petersen, John Pedersen
      Pages 229-243
  5. Various Applications of Affinity Chromatography

    1. Front Matter
      Pages 245-245

About this book


Thirty-eight years after its introduction, affinity chromatography remains a key tool in the armory of separation techniques available to separation and interaction scientists. Expanded and updated from the first edition, Affinity Chromatography: Methods and Protocols, Second Edition, provides the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era. This second edition expands on the first edition by introducing more state-of-the-art protocols used in affinity chromatography. This new edition also describes protocols that demonstrate the concept of affinity chromatography being applied to meet the modern high throughput screening demands of researchers and development scientists whilst expanding on some more traditional affinity chromatography approaches that have become of greater interest to separation scientists. Chapters in this cutting-edge text expand on affinity chromatography techniques that currently enjoy frequent citation in the literature from those purifying biomolecules. Other chapters include protocols describing the use of a variety of fusion tags as well as how to cleave them, so as to allow the scientists to study the native phenotype of the protein. Renowned researchers also include protocols detailing diverse applications of affinity chromatography such as its use in catalytic reactions, DNA purification, whole cell separations and for the isolation of phosphorylated proteins. Affinity Chromatography: Methods and Protocols, Second Edition, is an essential reference for those interested in separation sciences, particularly in the pharmaceutical and biological research sectors, that have an interest in isolating macromolecules rapidly, quantitatively, and with high purity.


Chromatography DNA Interaction science Post-genomic era Purification tags Separation techiques preparation protein proteins reaction

Editors and affiliations

  • Michael Zachariou
    • 1
  1. 1.BioMarin Pharmaceutical Inc.

Bibliographic information

  • Book Title Affinity Chromatography
  • Book Subtitle Methods and Protocols
  • Editors Michael Zachariou
  • Series Title Methods in Molecular Biology™
  • DOI
  • Copyright Information Humana Press 2008
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-58829-659-7
  • Softcover ISBN 978-1-61737-701-3
  • eBook ISBN 978-1-59745-582-4
  • Series ISSN 1064-3745
  • Edition Number 2
  • Number of Pages XII, 348
  • Number of Illustrations 47 b/w illustrations, 0 illustrations in colour
  • Topics Cell Biology
    Human Genetics
  • Buy this book on publisher's site
Industry Sectors
Health & Hospitals


From the reviews of the second edition:

"The book consists of three parts including 21 chapters that have been written by a large group of authors from many countries. … recommends this book as a manual for many specialists involved in purification of biomolecules in various fields of biochemistry, molecular biology, biotechnology, and bioorganic chemistry. The book can also be recommended as a handbook for university students and their teachers as well as for other schools preparing specialists in the above-mentioned fields." (G. Ya. Wiederschain, Biochemistry, November, 2008)