Gene Therapy of Cancer

Methods and Protocols

  • Wolfgang Walther
  • Ulrike S. Stein

Part of the Methods in Molecular Biology™ book series (MIMB, volume 542)

Table of contents

  1. Front Matter
    Pages ii-xiv
  2. Experimental Approaches in Cancer Gene Therapy: Introduction

    1. Front Matter
      Pages 1-2
    2. Jean-Pierre Gillet, Benjamin Macadangdang, Robert L. Fathke, Michael M. Gottesman, Chava Kimchi-Sarfaty
      Pages 5-54
  3. Vectorology for Cancer Gene Therapy

    1. Front Matter
      Pages 55-55
    2. Giulia Fulci, Brent Passer
      Pages 75-86
    3. Peter Mayrhofer, Martin Schleef, Wolfgang Jechlinger
      Pages 87-104
    4. John R. Ohlfest, Zoltán Ivics, Zsuzsanna Izsvák
      Pages 105-116
    5. Oleg Tolmachov
      Pages 117-129
  4. Nonviral Transfer Technologies in Cancer Gene Therapy

    1. Front Matter
      Pages 155-155
    2. Marco Schmeer
      Pages 156-164
    3. Kandan Aravindaram, Ning Sun Yang
      Pages 167-178
    4. Loreto B. Feril Jr.
      Pages 179-194
    5. Wolfgang Walther, Iduna Fichtner, Peter M. Schlag, Ulrike S. Stein
      Pages 195-208
  5. Experimental Studies in Cancer Gene Therapy

    1. Front Matter
      Pages 209-209
    2. Brian M. Olson, Douglas G. McNeel
      Pages 210-242
    3. Shuhong Han, Lung-Ji Chang
      Pages 245-259
    4. Natasa Zarovni, Riccardo Vago, Maria Serena Fabbrini
      Pages 261-283
    5. Jerry J. Jaboin, Zhaozhong Han, Dennis E. Hallahan
      Pages 285-300
    6. Chunsheng Kang, Peiyu Pu, Hao Jiang
      Pages 335-349
    7. Sunjoo Jeong, Hee Kyu Lee, Mee Young Kim
      Pages 363-377
    8. Paula J. Bates, Enid W. Choi, Lalitha V. Nayak
      Pages 379-392
  6. Clinical Approaches in Cancer Gene Therapy - Requirements for Clinical Gene Therapy Trials

    1. Front Matter
      Pages 393-394
    2. Carolyn M. Laurencot*, Sheryl Ruppel
      Pages 395-419
    3. Jonathan Kimmelman
      Pages 423-445
    4. Tiago Vicente, Cristina Peixoto, Manuel J.T. Carrondo, Paula M. Alves
      Pages 447-470
    5. Martin Schleef, Markus Blaesen
      Pages 471-495
  7. Clinical Protocols in Cancer Gene Therapy

    1. Front Matter
      Pages 497-497
    2. John J. J. Nemunaitis
      Pages 498-513
    3. Seunghee Kim-Schulze, Howard L. Kaufman
      Pages 515-527
    4. Luisa Barzon, Monia Pacenti, Elisa Franchin, Federico Colombo, Giorgio Palù
      Pages 529-549
    5. Chandini Thirukkumaran, Don G. Morris
      Pages 607-634
    6. Tulin Budak-Alpdogan, Joseph R. Bertino
      Pages 661-704
  8. Back Matter
    Pages 719-731

About this book


Despite various difficulties, the field of gene therapy, particularly with regard to cancer, has accumulated a tremendous amount of vital pre-clinical and clinical data. Gene Therapy of Cancer: Methods and Protocols, Second Edition fully updates the first edition with expert coverage of established and novel protocols involving both experimental and clinical approaches to cancer gene therapy. This state-of-the-art volume contains overviews of new concepts and strategies with chapters on regulatory and ethical issues, developments, problems and possible limitations of design and production of gene therapeutics as well as translational issues. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Cutting edge and authoritative, Gene Therapy of Cancer: Methods and Protocols, Second Edition is an ideal guide for all those who wish to explore the fast-paced and critical study of nonviral, viral, experimental and clinical cancer gene therapy.


Clinical protocols DNA Experimental studies Nonviral transfer Therapeutic genes Translation Vectorology gene therapy gene transfer genes molecular biology

Editors and affiliations

  • Wolfgang Walther
    • 1
  • Ulrike S. Stein
    • 2
  1. 1.Molecular Medicine (MDC)Max Delbrück Center forBerlinGermany
  2. 2.Molecular Medicine (MDC)Max Delbrück Center forBerlinGermany

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2009
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-934115-85-5
  • Online ISBN 978-1-59745-561-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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Health & Hospitals
Oncology & Hematology