Therapeutic Antibodies

Methods and Protocols

  • Antony S. Dimitrov

Part of the Methods in Molecular Biology™ book series (MIMB, volume 525)

Table of contents

  1. Front Matter
    Pages i-xviii
  2. Recombinant Antigens

    1. Front Matter
      Pages 1-1
    2. Yee-Peng Chan, Lianying Yan, Yan-Ru Feng, Christopher C. Broder
      Pages 31-58
  3. Antibody Libraries

    1. Front Matter
      Pages 1-1
    2. Weizao Chen, Zhongyu Zhu, Xiaodong Xiao, Dimiter S. Dimitrov
      Pages 81-99
  4. Antibody Discovery

    1. Front Matter
      Pages 1-1
    2. Mehdi Arbabi-Ghahroudi, Roger MacKenzie, Jamshid Tanha
      Pages 187-216
    3. Yariv Mazor, Thomas Van Blarcom, Brent L. Iverson, George Georgiou
      Pages 217-239
    4. Joanne Ayriss, Rosa Valero, Andrew R.M. Bradbury, Peter Pavlik
      Pages 241-260
    5. Per-Johan Meijer, Lars S. Nielsen, Johan Lantto, Allan Jensen
      Pages 261-277
    6. Brian R. Miller, Scott M. Glaser, Stephen J. Demarest
      Pages 279-289
  5. Antibody Engineering

    1. Front Matter
      Pages 1-1
    2. Holger Thie, Bernd Voedisch, Stefan Dübel, Michael Hust, Thomas Schirrmann
      Pages 309-322
    3. Jianbing Zhang, Roger MacKenzie, Yves Durocher
      Pages 323-336
    4. Mitchell Ho, Ira Pastan
      Pages 337-352
    5. Jenny Bostrom, Chingwei V. Lee, Lauric Haber, Germaine Fuh
      Pages 353-376
    6. Tim D. Jones, Laura J. Crompton, Frank J. Carr, Matthew P. Baker
      Pages 405-423
    7. Dianne L. Newton, Luke H. Stockwin, Susanna M. Rybak
      Pages 425-443
    8. Rajeeva Singh, Hans K. Erickson
      Pages 445-467
  6. Antibody Preclinical Development

    1. Front Matter
      Pages 1-1
    2. Jürgen Krauss, Evelyn Exner, Athanasios Mavratzas, Siegfried Seeber, Michaela A.E. Arndt
      Pages 471-490
    3. Dimana Dimitrova, Vidita Choudhry, Christopher C. Broder
      Pages 491-498
    4. Eva Jordan, Laila Al-Halabi, Thomas Schirrmann, Michael Hust
      Pages 509-516
    5. Ilia Prado, Timothy R. Fouts, Antony S. Dimitrov
      Pages 517-531
    6. Bang K. Vu, Joseph Walsh, Dimiter S. Dimitrov, Rieko Ishima
      Pages 533-543
  7. Antibody Sequence Analysis

  8. Back Matter
    Pages 581-593

About this book


With revenues from the top five therapeutic antibodies accounting for a majority of the recent pharmaceutical sales, the research and development in the field has exploded over the past several years and is expected to grow with new emerging monoclonal antibodies like Numax, Lucentis, Actemra, and others. In Therapeutic Antibodies: Methods and Protocols, leading experts from academic laboratories and biotechnology companies present an extensive set of protocols for the discovery and development of therapeutic antibodies, featuring sections devoted to recombinant antigens, antibody libraries, antibody discovery, antibody engineering, and antibody preclinical development. Written in the highly successful Methods in Molecular Biology™ series format, the chapters contain brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes highlighting tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Therapeutic Antibodies: Methods and Protocols serves as a key resource for researchers and antibody engineers investigating and participating in this rapidly growing therapeutic market segment.


Antibody discovery Antibody engineering Antibody libraries Antibody preclinical development Antigen HIV Monoclonal antibodies Recombinant antigens dynamics

Editors and affiliations

  • Antony S. Dimitrov

There are no affiliations available

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2009
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-934115-92-3
  • Online ISBN 978-1-59745-554-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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