Reporter Genes

A Practical Guide

  • Donald S. Anson

Part of the Methods in Molecular Biology book series (MIMB, volume 411)

Table of contents

  1. Front Matter
    Pages i-x
  2. Maria Limberis, Peter Bell, James M. Wilson
    Pages 25-34
  3. George H. Patterson
    Pages 47-80
  4. Karin Edoff, James S. Dods, Andrea H. Brand
    Pages 81-98
  5. Charles G. Bailey, John E. J. Rasko
    Pages 99-110
  6. Steven K. Dower, Eva E. Qwarnstrom, Endre Kiss-Toth
    Pages 111-119
  7. Robert M. Hoffman
    Pages 121-129
  8. Harald Jockusch, Daniel Eberhard
    Pages 145-154
  9. Back Matter
    Pages 155-158

About this book

Introduction

Reporter genes have played, and continue to play, a vital role in many areas of biological research by providing a ready means for qualitative and quantitative assessment of the activity of genes and location of gene products in different environments. In Reporter Genes: A Practical Guide, renowned researchers describe practical protocols for experimentation with the most useful reporter genes for mammalian systems that are available, concentrating on those marker genes that are currently most commonly used. Among the topics covered in this volume are methodologies for staining and visualization of ß-galactosidase in embryos and tissues, immunohistochemical detection of ß-galactosidase, detection of reporter gene expression in murine airways, and three dimensional analysis of molecular signals with episcopic imaging techniques. Also covered in this groundbreaking text are fluorescent proteins, detection of GFP during nervous system development, fluorescent protein reporter systems for single cell measurements, and non-invasive imaging of molecular events. Comprehensive and illuminating, Reporter Genes: A Practical Guide will be an oft-used reference for geneticists, molecular engineers, and molecular biologists.

Keywords

Gene activity Mammalian systems Marker genes Reporter gene expression cell biology gene expression genes proteins

Editors and affiliations

  • Donald S. Anson
    • 1
  1. 1.Department of Genetic MedicineWomen’s and Children’s HospitalAdelaideAustralia

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-59745-549-7
  • Copyright Information Humana Press Inc. 2007
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-739-6
  • Online ISBN 978-1-59745-549-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book